Optimization of peptide separations by differential ion mobility spectrometry.
Optimization of peptide separations by differential ion mobility spectrometry.
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DOI：
10.1007/s13361-014-0941-9
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发表时间：
2014-09
影响因子：
3.2
通讯作者：
Glish, Gary L.
中科院分区：
文献类型：
--
作者：
Isenberg, Samantha L.;Armistead, Paul M.;Glish, Gary L.
关键词：
差分离子迁移谱法（DIMS）具有基于它们在低电场和高电场中的离子迁移率的差异来分离气相离子的能力。DIMS可用于分离质谱法（MS）无法区分的同量异位素和异构体物质的混合物。DIMS也可用作过滤器，以改善复杂样品中分析物的信号背景比。DIMS分离的分辨能力可以通过几种方式来提高，包括增加色散场和增加氮气载气中氦气的量。先前已经证明，当分散场随着氦含量变化而保持恒定时，向DIMS载气中添加氦提供了改进的分离。然而，氦具有比氮低的击穿电压。因此，随着氮气载气中氦含量的百分比增加，可达到的最高色散场减小。这项工作提出了增加分散场和使用氦气的载气之间的权衡，通过比较同量异位素肽的混合物的分离。通过在DIMS组件内使用高分散场（~72 kV/cm）和纯氮气作为载气，实现了具有相同标称摩尔质量的三种肽的混合物的分离的最大分辨率。用于实现最大分辨率的条件也表现出通过组件的最低离子传输，这表明当针对给定应用优化DIMS条件时，有必要考虑灵敏度和分辨率之间的权衡。
Differential ion mobility spectrometry (DIMS) has the ability to separate gas phase ions based on their difference in ion mobility in low and high electric fields. DIMS can be used to separate mixtures of isobaric and isomeric species indistinguishable by mass spectrometry (MS). DIMS can also be used as a filter to improve the signal-to-background of analytes in complex samples. The resolving power of DIMS separations can be improved several ways, including increasing the dispersion field and increasing the amount of helium in the nitrogen carrier gas. It has been previously demonstrated that the addition of helium to the DIMS carrier gas provides improves separations when the dispersion field is the kept constant as helium content is varied. However, helium has a lower breakdown voltage than nitrogen. Therefore, as the percent helium content in the nitrogen carrier gas is increased, the highest dispersion field accessible decreases. This work presents the trade-offs between increasing dispersion fields and using helium in the carrier gas by comparing the separation of a mixture of isobaric peptides. The maximum resolution for a separation of a mixture of three peptides with the same nominal molar mass was achieved by using a high dispersion field (~72 kV/cm) with pure nitrogen as the carrier gas within the DIMS assembly. The conditions used to achieve the maximum resolution also exhibit the lowest ion transmission through the assembly, suggesting that it is necessary to consider the trade-off between sensitivity and resolution when optimizing DIMS conditions for a given application.
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影响因子：
7.4
作者：
Shvartsburg, Alexandre A.;Smith, Richard D.
通讯作者：
Smith, Richard D.
影响因子：
7.4
作者：
Shvartsburg, Alexandre A.;Prior, David C.;Tang, Keqi;Smith, Richard D.
通讯作者：
Smith, Richard D.
影响因子：
7.4
作者：
Shvartsburg, AA;Tang, KQ;Smith, RD
通讯作者：
Smith, RD
影响因子：
2
作者：
Hatsis, Panos;Valaskovic, Gary;Wu, Jing-Tao
通讯作者：
Wu, Jing-Tao
影响因子：
1.6
作者：
Purves, RW;Guevremont, R;Matyjaszczyk, MS
通讯作者：
Matyjaszczyk, MS