Tissue-Specific Expression of Circ_015343 and Its Inhibitory Effect on Mammary Epithelial Cells in Sheep.
Tissue-Specific Expression of Circ_015343 and Its Inhibitory Effect on Mammary Epithelial Cells in Sheep.
复制标题
DOI：
10.3389/fvets.2022.919162
复制
发表时间：
2022
影响因子：
3.2
通讯作者：
中科院分区：
文献类型：
--
作者：
环状RNA（circular RNA）是一类在哺乳动物乳腺发育和泌乳过程中具有重要分子功能的非编码RNA。在我们之前的研究中，通过使用RNA测序（RNA-seq）发现circ_015343在泌乳高峰期的绵羊乳腺组织中高度表达。本研究通过逆转录-聚合酶链反应（RT-PCR）分析和桑格测序证实了circ_015343的真实性。circ_015343来源于氨基己二酸半醛合酶（aminoadipic semialdehyde synthase，AASS）基因的10个外显子，从外显子2到外显子11，主要定位于绵羊乳腺上皮细胞的胞质中。circRNA在绵羊8种组织中均有表达，其中乳腺组织中表达量最高，背最长肌组织中表达量最低。circ_015343在具有较高产奶量和乳脂含量的绵羊品种中具有较低的表达水平。干扰的circ_015343增加了绵羊乳腺上皮细胞的活力和增殖。circ_015343的抑制还增加了三种乳脂合成标记基因的表达水平：乙酰辅酶A羧化酶α（ACACA）、脂肪酸结合蛋白4（FABP 4）和固醇调节元件结合蛋白1（SREBP 1），以及三种增殖相关基因：细胞周期蛋白依赖性激酶2（CDK 2）、细胞周期蛋白依赖性激酶4（CDK 4）和增殖细胞核抗原（PCNA）的表达，但降低其亲本基因AASS的表达水平。circRNA-miRNA-mRNA相互作用网络显示，circ_015343会结合一些microRNA（miRNAs），调控与乳腺发育和泌乳相关的功能基因的表达。本研究有助于更好地了解circ_015343在绵羊乳腺中的作用。
Circular RNAs (circRNAs) are a kind of non-coding RNA that have an important molecular function in mammary gland development and lactation of mammals. In our previous study, circ_015343 was found to be highly expressed in the ovine mammary gland tissue at the peak-lactation period by using RNA sequencing (RNA-seq). In the present study, the authenticity of circ_015343 was confirmed by using reverse transcriptase-polymerase chain reaction (RT-PCR) analysis and Sanger sequencing. The circ_015343 was derived from the complete 10 exons of aminoadipic semialdehyde synthase (AASS), ranging from exon 2 to exon 11 and mainly located in cytoplasm of ovine mammary epithelial cells. The circRNA was found to be expressed in eight ovine tissues, with the highest expression level in the mammary gland and the least expression in Longissimus dorsi muscle. The circ_015343 had a lower level of expression in a sheep breed with higher milk yield and milk fat content. The disturbed circ_015343 increased the viability and proliferation of the ovine mammary epithelial cells. The inhibition of circ_015343 also increased the expression levels of three milk fat synthesis marker genes: acetyl-coenzyme A carboxylase alpha (ACACA), fatty acid-binding protein 4 (FABP4), and sterol regulatory element-binding protein 1 (SREBP1), as well as three proliferation-related genes: cyclin dependent kinase 2 (CDK2), cyclin dependent kinase 4 (CDK4) and proliferating cell nuclear antigen (PCNA), but decreased the expression level of its parent gene AASS. A circRNA-miRNA-mRNA interaction network showed that circ_015343 would bind some microRNAs (miRNAs) to regulate the expression of functional genes related to the development of mammary gland and lactation. This study contributes to a better understanding of the roles of circ_015343 in the mammary gland of sheep.
登录
查看更多内容
影响因子：
5.6
作者：
Li N;Zhao F;Wei C;Liang M;Zhang N;Wang C;Li QZ;Gao XJ
通讯作者：
Gao XJ
影响因子：
3.7
作者：
Nagaoka K;Zhang H;Watanabe G;Taya K
通讯作者：
Taya K
MicroRNA-106b 通过 ATP 结合盒亚家族 A 成员 1 (ABCA1) 调节牛乳腺上皮细胞中的乳脂代谢
影响因子：
6.1
作者：
Chen, Zhi;Chu, Shuangfeng;Yang, Zhangping
通讯作者：
Yang, Zhangping
DOI：
10.1016/j.bbrc.2019.03.022
发表时间：
2019-04-30
影响因子：
3.1
作者：
Le Guillou, Sandrine;Laubier, Johann;Le Provost, Fabienne
通讯作者：
Le Provost, Fabienne
影响因子：
4.5
作者：
Pink, Desmond B. S.;Gatrell, Stephanie K.;Ball, Ronald O.
通讯作者：
Ball, Ronald O.