Expression, Purification, and Antiserum Production of the Truncated UL31 Protein of Herpes Simplex Virus 1
Expression, Purification, and Antiserum Production of the Truncated UL31 Protein of Herpes Simplex Virus 1
复制标题
单纯疱疹病毒 1 型截短 UL31 蛋白的表达、纯化和抗血清生产
DOI：
10.21859/ijb.1609
复制
发表时间：
2019-01
影响因子：
1.3
通讯作者：
Mingsheng Cai
中科院分区：
文献类型：
--
作者：
Xingmei Zou;Zuo Xu;Yuanfang Wang;Xiaowen Ou;Yiwen Li;Delong Liu;Weidong Gan;Manjiao Lu;Qiusan Chen;Hao Peng;Gengde Hong;Jinyu Lin;Meili Li;Mingsheng Cai
背景
单纯疱疹病毒1型（HSV - 1）的UL31蛋白在HSV - 1复制中起重要作用，然而，其在病毒生命周期中的精确功能尚未得到充分阐明。
目的
一种用于检测的特异性抗血清……
Background The UL31 protein of herpes simplex virus 1 (HSV-1) plays an important role in the HSV-1 replication, however, its pinpoint functions in the life cycle of the virus have yet to be adequately elucidated. Objectives An antiserum specific for detecting HSV-1 UL31 was prepared as the foundation for future research on the role of UL31 in the course of HSV-1 infection. Materials and Methods Recombinant protein of UL31 was expressed in Escherichia coli, which was then purified and employed to raise the level of antiserum in mice. Subsequently, western blot and immunofluorescence assay (IFA) were utilized to detect the specific antiserum. Results The recombinant UL31 protein consisting of N-terminal 27 aa of UL31 was fused to EYFP and His-tag. It was expressed, purified, and applied to the preparation of the antiserum. Western blot analysis and IFA demonstrated that this antiserum could detect both the recombinant UL31 and the native UL31. Conclusions Our results manifest that this antiserum could be conducive to further investigations concerning the roles of UL31 in the HSV-1 infection.
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