Remodeling of monoplanar Purkinje cell dendrites during cerebellar circuit formation.
Remodeling of monoplanar Purkinje cell dendrites during cerebellar circuit formation.
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DOI：
10.1371/journal.pone.0020108
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发表时间：
2011
期刊：
影响因子：
3.7
通讯作者：
Kengaku M
中科院分区：
文献类型：
--
作者：
Kaneko M;Yamaguchi K;Eiraku M;Sato M;Takata N;Kiyohara Y;Mishina M;Hirase H;Hashikawa T;Kengaku M
树突树枝化模式是神经元连接和整合的关键决定因素。小脑浦肯野细胞的平面和高度分支的树突从两个主要的传入通路接收特定的地形投影;从下橄榄沿着浦肯野树突延伸的单个攀爬纤维轴突，以及垂直于树突平面接触的颗粒细胞的平行纤维轴突。据认为，鼠浦肯野细胞树突在出生后早期发育过程中细胞极性确定后，在分子层中沿单个旁视平面延伸。通过对生长中的浦肯野细胞的三维共聚焦分析，我们观察到小鼠浦肯野细胞在出生后第三周的环路成熟过程中经历了动态树突重塑。在出生后第二周早期，树突被极化和扁平化后，到出生后第三周，树突乔木通过密集的生长和分支在分子层的多个矢状面中逐渐扩展。在出生后的第四周，树突被限制在一个平面上。多平面浦肯野细胞在第三周往往与异位攀缘纤维支配附近的浦肯野细胞在不同的矢状面。在浦肯野细胞连接异常和运动不协调的突变小鼠中，成熟的单平面分支被破坏。在出生后第二或第三周，正常传入活动的药理学破坏也损害了树突重塑。我们的研究结果表明，浦肯野细胞的单平面分支与小脑电路的功能发展相结合。
Dendrite arborization patterns are critical determinants of neuronal connectivity and integration. Planar and highly branched dendrites of the cerebellar Purkinje cell receive specific topographical projections from two major afferent pathways; a single climbing fiber axon from the inferior olive that extend along Purkinje dendrites, and parallel fiber axons of granule cells that contact vertically to the plane of dendrites. It has been believed that murine Purkinje cell dendrites extend in a single parasagittal plane in the molecular layer after the cell polarity is determined during the early postnatal development. By three-dimensional confocal analysis of growing Purkinje cells, we observed that mouse Purkinje cells underwent dynamic dendritic remodeling during circuit maturation in the third postnatal week. After dendrites were polarized and flattened in the early second postnatal week, dendritic arbors gradually expanded in multiple sagittal planes in the molecular layer by intensive growth and branching by the third postnatal week. Dendrites then became confined to a single plane in the fourth postnatal week. Multiplanar Purkinje cells in the third week were often associated by ectopic climbing fibers innervating nearby Purkinje cells in distinct sagittal planes. The mature monoplanar arborization was disrupted in mutant mice with abnormal Purkinje cell connectivity and motor discoordination. The dendrite remodeling was also impaired by pharmacological disruption of normal afferent activity during the second or third postnatal week. Our results suggest that the monoplanar arborization of Purkinje cells is coupled with functional development of the cerebellar circuitry.
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