Maternal high-fat diet during gestation or suckling differentially affects offspring leptin sensitivity and obesity.
Maternal high-fat diet during gestation or suckling differentially affects offspring leptin sensitivity and obesity.
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DOI：
10.2337/db11-0957
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发表时间：
2012-11
期刊：
影响因子：
7.7
通讯作者：
Tamashiro KL
中科院分区：
文献类型：
--
作者：
Sun B;Purcell RH;Terrillion CE;Yan J;Moran TH;Tamashiro KL
母体在整个妊娠期和哺乳期的高脂肪（HF）饮食对后代的代谢表型具有长期影响。在这里，我们确定了产前或产后母亲HF饮食对后代代谢表型的相对贡献。在整个妊娠和哺乳期间，妊娠Sprague-Dawley大鼠维持正常饲料或HF饲料。在出生后第1天（PND），将所有窝仔与饲料或HF母鼠交叉饲养，形成4组。在断奶和成年期测量体重、身体组成和葡萄糖耐量。在PND 10和PND 21通过信号转导和转录激活因子（STAT）3激活评估瘦素敏感性。到PND 7，与HF母鼠交叉寄养的幼仔的体重增加量大于进食幼仔，并持续至断奶。出生后的HF幼崽有更大的肥胖，更高的血浆瘦素浓度，糖耐量受损，并减少磷酸化STAT3响应瘦素在弓状核断奶。断奶后，与HF母鼠交叉饲养的雄性后代摄食量高，体重保持高于出生后的食物幼仔。哺乳期的出生后HF饮食继续损害成年期雄性和雌性后代的葡萄糖耐量。母乳喂养期间的母亲HF饮食在确定后代的代谢表型方面比产前HF饮食暴露具有更大的影响，并且可以为母亲和儿童提供关于最佳围产期营养的见解。
Maternal high-fat (HF) diet throughout gestation and suckling has long-term consequences on the offspring’s metabolic phenotype. Here we determine the relative contribution of pre- or postnatal maternal HF diet on offspring’s metabolic phenotype. Pregnant Sprague-Dawley rats were maintained on normal chow or HF diet throughout gestation and suckling. All litters were cross-fostered to chow or HF dams on postnatal day (PND)1, resulting in four groups. Body weight, body composition, and glucose tolerance were measured at weaning and in adulthood. Leptin sensitivity was assessed by signal transducer and activator of transcription (STAT)3 activation on PND10 and PND21. Pups cross-fostered to HF dams gained more body weight than chow pups by PND7 and persisted until weaning. Postnatal HF pups had greater adiposity, higher plasma leptin concentration, impaired glucose tolerance, and reduced phosphorylated STAT3 in response to leptin in the arcuate nucleus at weaning. After weaning, male offspring cross-fostered to HF dams were hyperphagic and maintained greater body weight than postnatal chow pups. Postnatal HF diet during suckling continued to impair glucose tolerance in male and female offspring in adulthood. Maternal HF diet during suckling has a greater influence in determining offspring’s metabolic phenotype than prenatal HF diet exposure and could provide insight regarding optimal perinatal nutrition for mothers and children.
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影响因子：
7.1
作者：
Biro, Frank M.;Wien, Michelle
通讯作者：
Wien, Michelle
影响因子：
4.2
作者：
Fahrenkrog, S;Harder, T;Plagemann, A
通讯作者：
Plagemann, A
影响因子：
5.3
作者：
Hübschle, T;Thom, E;Meyerhof, W
通讯作者：
Meyerhof, W
影响因子：
2.2
作者：
Cravo, CD;Teixeira, CV;Ramos, C
通讯作者：
Ramos, C
影响因子：
4.9
作者：
Hofbauer, K
通讯作者：
Hofbauer, K