Oligothiophenes as fluorescent markers for biological applications.
Oligothiophenes as fluorescent markers for biological applications.
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寡聚型碘作为生物应用的荧光标记。
DOI：
10.3390/molecules17010910
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发表时间：
2012-01-18
期刊：
影响因子：
--
通讯作者：
Manetto A
中科院分区：
文献类型：
--
作者：
Capobianco ML;Barbarella G;Manetto A
本文总结了我们在寡聚噻吩作为荧光标记物在生物学研究中的应用方面的一些结果。噻吩的低聚物因其在有机电子学中的半导体性质而广为人知，也是以化学和光学稳定性、高吸光度和量子产率为特征的荧光化合物。它们的荧光发射可以通过有机合成通过改变噻吩环的数目和侧链的性质容易地调制。本文综述了寡聚噻吩类化合物如何与亚磷酰胺、N-羟基琥珀酰亚胺基和4-磺基四氟苯基酯、异硫氰酸酯和叠氮化物等活性基团进行衍生化，从而使感兴趣的（生物）分子共价结合。本文还介绍了如何分子，如寡核苷酸，蛋白质，甚至纳米粒子，标记寡噻吩，可用于从杂交研究到固定和活细胞成像的实验。最后，一些多标记实验进行了说明。
This paper summarizes some of our results on the application of oligothiophenes as fluorescent markers for biological studies. The oligomers of thiophene, widely known for their semiconductor properties in organic electronics, are also fluorescent compounds characterized by chemical and optical stability, high absorbance and quantum yield. Their fluorescent emission can be easily modulated via organic synthesis by changing the number of thiophene rings and the nature of side-chains. This review shows how oligothiophenes can be derivatized with active groups such as phosphoramidite, N-hydroxysuccinimidyl and 4-sulfotetrafluorophenyl esters, isothiocyanate and azide by which the (bio)molecules of interest can be covalently bound. This paper also describes how molecules such as oligonucleotides, proteins and even nanoparticles, tagged with oligothiophenes, can be used in experiments ranging from hybridization studies to imaging of fixed and living cells. Finally, a few multilabeling experiments are described.
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影响因子：
3.6
作者：
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通讯作者：
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