Anti-quorum sensing and anti-biofilm activity of nickel oxide nanoparticles against Pseudomonas aeruginosa

Anti-quorum sensing and anti-biofilm activity of nickel oxide nanoparticles against Pseudomonas aeruginosa
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氧化镍纳米颗粒对铜绿假单胞菌的抗群体感应和抗生物膜活性

DOI:
10.1016/j.jece.2020.104533
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发表时间:
2020-12
影响因子:
7.7
通讯作者:
Wen-Jun Li
Wen-Jun Li
中科院分区:
工程技术2区
文献类型:
--
作者:
Muthuchamy Maruthup;y;Govindan Nadar Rajivg;hi;Franck Quero;Wen-Jun Li

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采用化学还原法制备了氧化镍纳米粒子(NiO NPs)。通过粉末 X 射线衍射、热重分析、傅里叶变换红外光谱、紫外可见漫反射光谱、扫描电子显微镜和透射电子显微镜对 NiO 纳米颗粒的物理和形态观察进行了评估。此外,紫罗兰色杆菌 ATCC 12472 中紫罗兰素降解的体外抑制研究以及紫罗兰色杆菌 ATCC 12472 中生物膜根除的体外研究。检测到的铜绿假单胞菌浓度分别为 50 μg/mL 和 60 μg/mL。此外,通过环生物膜降解和时间杀灭变异测定证实了生物膜抑制。使用共焦激光扫描显微镜和扫描电镜研究了 NiO NP 的活/死细胞变化、生物膜结构损伤和细胞壁修饰。此外,NiO NPs被发现可以作为抗P的生物膜剂。铜绿假单胞菌。最后,研究了 NiO NPs 对卤虫的体内毒性,表明毒性较低。
Nickel oxide nanoparticles (NiO NPs) were prepared by a chemical reduction method. The physical and morphological observations of NiO NPs were assessed by powder X-ray diffraction, thermogravimetric analysis, Fourier transform infrared spectroscopy, and UV–vis diffuse reflectance spectroscopy, scanning electron microscopy and transmission electron microscopy. Furthermore, thein vitroinhibition studies of violacein degradation inChromobacterium violaceumATCC 12472 and biofilm eradication inP. aeruginosawere detected at 50 μg/mL and 60 μg/mL, respectively. In addition, the biofilm inhibition was confirmed by ring biofilm degradation and time-kill variation assays. The live/dead cell variations, biofilm architecture damage and cell wall modifications of NiO NPs were investigated using confocal laser scanning microscope and SEM. In addition, NiO NPs were found to act as anti-biofilm agent againstP. aeruginosa. Finally thein-vivotoxicity of NiO NPs was investigated againstArtemia franciscana, suggesting low toxicity.
一个有效的系统,用于β-内酰胺抗生素的细胞内递送以克服细菌耐药性。
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