Spatial detection of fetal marker genes expressed at low level in adult human heart tissue.
Spatial detection of fetal marker genes expressed at low level in adult human heart tissue.
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DOI：
10.1038/s41598-017-13462-5
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发表时间：
2017-10-11
影响因子：
4.6
通讯作者：
Lundeberg J
中科院分区：
文献类型：
--
作者：
Asp M;Salmén F;Ståhl PL;Vickovic S;Felldin U;Löfling M;Fernandez Navarro J;Maaskola J;Eriksson MJ;Persson B;Corbascio M;Persson H;Linde C;Lundeberg J
Heart failure is a major health problem linked to poor quality of life and high mortality rates. Hence, novel biomarkers, such as fetal marker genes with low expression levels, could potentially differentiate disease states in order to improve therapy. In many studies on heart failure, cardiac biopsies have been analyzed as uniform pieces of tissue with bulk techniques, but this homogenization approach can mask medically relevant phenotypes occurring only in isolated parts of the tissue. This study examines such spatial variations within and between regions of cardiac biopsies. In contrast to standard RNA sequencing, this approach provides a spatially resolved transcriptome- and tissue-wide perspective of the adult human heart, and enables detection of fetal marker genes expressed by minor subpopulations of cells within the tissue. Analysis of patients with heart failure, with preserved ejection fraction, demonstrated spatially divergent expression of fetal genes in cardiac biopsies.
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DOI：
10.1093/bioinformatics/btu638
发表时间：
2015-01-15
期刊：
Bioinformatics (Oxford, England)
影响因子：
--
作者：
Anders S;Pyl PT;Huber W
通讯作者：
Huber W
返回到胎儿基因程序：建议与心脏中基因表达的代谢联系。
影响因子：
5.2
作者：
Taegtmeyer H;Sen S;Vela D
通讯作者：
Vela D
影响因子：
3.7
作者：
Lundin, Sverker;Stranneheim, Henrik;Lundeberg, Joakim
通讯作者：
Lundeberg, Joakim
影响因子：
5.9
作者：
Bedada, Fikru B.;Chan, Sunny S-K.;Metzger, Stefania K.;Zhang, Liying;Zhang, Jianyi;Garry, Daniel J.;Kamp, Timothy J.;Kyba, Michael;Metzger, Joseph M.
通讯作者：
Metzger, Joseph M.
影响因子：
20.1
作者：
Roger VL
通讯作者：
Roger VL