The promotion of in vitro vessel-like organization of endothelial cells in magnetically responsive alginate scaffolds.
The promotion of in vitro vessel-like organization of endothelial cells in magnetically responsive alginate scaffolds.
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DOI：
10.1016/j.biomaterials.2012.02.037
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发表时间：
2012-06
期刊：
影响因子：
14
通讯作者：
Polyak, Boris
中科院分区：
文献类型：
--
作者：
Sapir, Yulia;Cohen, Smadar;Friedman, Gary;Polyak, Boris
关键词：
在设计厚重、复杂的组织(如心肌)方面，主要挑战之一是需要在体外对工程组织进行预血管处理，以使其在植入后能够有效地与宿主组织整合。在此，我们探索了新型磁性海藻酸盐复合支架，为细胞提供物理刺激的手段。在总共14天的实验过程中的前7天，种植有主动脉内皮细胞的磁铁矿浸渍的藻酸盐支架在刺激期间表现出显著的代谢活性增加。增殖细胞核抗原的表达表明，磁刺激细胞的增殖指数低于未刺激的细胞。这表明，代谢活动的增加可能与细胞迁移和重组有关。免疫染色和共聚焦显微镜分析支持这一观察结果，在第14天，在没有补充任何生长因子的磁刺激支架中，与未刺激(对照)支架中观察到的细胞片或聚集体相比，形成了被称为血管生成和血管生成指示物的细胞血管样(环)结构。这项工作是我们理解如何准确控制细胞组织以形成组织工程化构建物的第一步，这种构建物与额外的分子信号一起可能导致创建一种高效的预血管组织构建物，具有潜在的移植应用价值。
One of the major challenges in engineering thick, complex tissues such as cardiac muscle, is the need to pre-vascularize the engineered tissue in vitro to enable its efficient integration with host tissue upon implantation. Herein, we explored new magnetic alginate composite scaffolds to provide means of physical stimulation to cells. Magnetite-impregnated alginate scaffolds seeded with aortic endothelial cells stimulated during the first 7 days out of a total 14 day experimental course showed significantly elevated metabolic activity during the stimulation period. Expression of proliferating cell nuclear antigen (PCNA) indicated that magnetically stimulated cells had a lower proliferation index as compared to the non-stimulated cells. This suggests that the elevated metabolic activity could instead be related to cell migration and re-organization. Immunostaining and confocal microscopy analyses supported this observation showing that on day 14 in magnetically stimulated scaffolds without supplementation of any growth factors, cellular vessel-like (loop) structures, known as indicators of vasculogenesis and angiogenesis were formed as compared to cell sheets or aggregates observed in the non-stimulated (control) scaffolds. This work is the first step in our understanding of how to accurately control cellular organization to form tissue engineered constructs, which together with additional molecular signals could lead to a creation of an efficient pre-vascularized tissue construct with potential applicability for transplantation.
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影响因子：
5.8
作者：
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通讯作者：
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影响因子：
3.9
作者：
Liu, Ting-Yu;Hu, Shang-Hsiu;Chen, San-Yuan
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影响因子：
3.7
作者：
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通讯作者：
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影响因子：
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作者：
Bhana, Bashir;Iyer, Rohin K.;Radisic, Milica
通讯作者：
Radisic, Milica
DOI：
10.1111/j.1749-6632.2002.tb03111.x
发表时间：
2002-01-01
期刊：
REPARATIVE MEDICINE: GROWING TISSUES AND ORGANS
影响因子：
--
作者：
Guldberg, RE
通讯作者：
Guldberg, RE