Pathological Change and Whole Transcriptome Alternation Caused by ePTFE Implantation in Myocardium.
Pathological Change and Whole Transcriptome Alternation Caused by ePTFE Implantation in Myocardium.
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ePTFE植入引起的心肌病理变化及全转录组改变
DOI：
10.1155/2021/5551207
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发表时间：
2021
影响因子：
--
通讯作者：
Jia B
中科院分区：
文献类型：
--
作者：
Shan Y;Zhang W;Chen G;Shi Q;Mi Y;Zhang H;Jia B
膨胀型聚四氟乙烯（ePTFE）常用于心血管手术，但常引起术后并发症。虽然人们已经做了大量的努力来缓解这些并发症或了解其机制，但没有可用的策略，也没有了解机制，特别是在心肌中。在此，将ePTFE膜植入家兔的右心室流出道，解剖植入相关心肌，并通过组织学和转录组测序进行分析。ePTFE植入导致心肌炎症和纤维化。鉴定出1867种不同表达的mRNA（DEmRNA，1107种上调和760种下调）和246种不同表达的lncRNA（DElncRNA，110种上调和136种下调）。生物信息学分析表明，上调的DEmRNAs和DElncRNAs主要参与炎症、免疫反应和细胞外基质重塑，而下调的DEmRNAs和DElncRNAs主要参与代谢和心脏重塑。对DEmRNAs和DElncRNAs的共表达和调控关系的分析表明，大多数DElncRNAs对相关DEmRNAs进行反式调节。总之，ePTFE植入导致严重的心肌组织损伤，并改变了心肌的转录组谱。这些新的数据可以提供ePTFE植入引起的不良反应的机制，并发现抑制ePTFE相关并发症的新的治疗靶点。
Expanded polytetrafluoroethylene (ePTFE) is commonly used in cardiovascular surgery, but usually causes postoperation complications. Although great efforts have been done to relieve these complications or to understand their mechanism, there are no applicable strategies available and no understanding mechanisms, especially in the myocardium. Here, ePTFE membranes are implanted into the right ventricular outflow tract of rabbits, and the implant-related myocardium is dissected and analyzed by histology and transcriptome sequencing. ePTFE implantation causes myocardium inflammation and fibrosis. There are 1867 differently expressed mRNAs (DEmRNAs, 1107 upregulated and 760 downregulated) and 246 differently expressed lncRNAs (DElncRNAs, 110 upregulated and 136 downregulated) identified. Bioinformatic analysis indicates that the upregulated DEmRNAs and DElncRNAs are mainly involved in inflammatory, immune responses, and extracellular matrix remodeling, while the downregulated DEmRNAs and DElncRNAs are predominantly functioned in the metabolism and cardiac remodeling. Analysis of coexpression and regulatory relationship of DEmRNAs and DElncRNAs reveals that most DElncRNAs are trans-regulated on the relevant DEmRNAs. In conclusion, ePTFE implantation causes severe myocardial tissue damages and alters the transcriptome profiles of the myocardium. Such novel data may provide a landscape of mechanisms underlying the adverse reactions caused by ePTFE implantation and uncover new therapeutic targets for inhibiting the ePTFE-related complications.
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影响因子：
6.2
作者：
Jansen LE;Amer LD;Chen EY;Nguyen TV;Saleh LS;Emrick T;Liu WF;Bryant SJ;Peyton SR
通讯作者：
Peyton SR
涂有抗 CD133 抗体功能化肝素/胶原多层且具有快速体内内皮化特性的合成 ePTFE 移植物
影响因子：
9.5
作者：
Lu, Shuyang;Zhang, Peng;Wang, Chunsheng
通讯作者：
Wang, Chunsheng
影响因子：
4.6
作者：
Lee, Jae Sang;Shin, Byung Ho;Koh, Won-Gun
通讯作者：
Koh, Won-Gun
DOI：
10.1016/j.jconrel.2018.09.021
发表时间：
2018-11-10
期刊：
Journal of controlled release : official journal of the Controlled Release Society
影响因子：
--
作者：
Gu B;Papadimitrakopoulos F;Burgess DJ
通讯作者：
Burgess DJ
影响因子：
15.9
作者：
Jain, Manish;Dhanesha, Nirav;Chauhan, Anil K.
通讯作者：
Chauhan, Anil K.