Observation of the Epithelial Cell Behavior in the Nasal Septum During Primary Palate Closure in Mice.
Observation of the Epithelial Cell Behavior in the Nasal Septum During Primary Palate Closure in Mice.
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DOI：
10.3389/fphys.2020.538835
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发表时间：
2020
影响因子：
4
通讯作者：
Yamashiro T
中科院分区：
文献类型：
--
作者：
Yamamoto S;Kurosaka H;Miura J;Aoyama G;Sarper SE;Oka A;Inubushi T;Nakatsugawa K;Usami Y;Toyosawa S;Yamashiro T
上皮融合在腭裂发生中起着至关重要的作用，而不完全融合会导致各种类型的面部裂隙，这取决于未能融合的区域。在哺乳动物的腭裂发生过程中，两侧的次级腭突在面部中部融合形成次级腭突。随后，次级腭架的背侧与鼻中隔融合，完成腭裂的形成。重要的是，次级腭架的前缘与位于鼻中隔前缘和腹缘的初级腭融合。虽然许多研究已经研究了次级腭架之间的融合机制，但对初级腭部如何与次级腭架接触和融合知之甚少。在这项研究中，我们使用K14-GFP小鼠的腭部外植体培养来研究初级腭部表面可能的上皮细胞行为。GFP标记的上皮细胞的时间推移观察和扫描电子显微镜分析表明，突起上皮出现在融合区对应的区域，并在没有次级腭突的情况下在鼻中隔表面呈吻状扩张。与次级腭面不同，细胞迁移和随后的自主性间充质暴露在鼻中隔或初级腭部不明显。TUNEL染色显示这些脱出的上皮细胞正在发生凋亡。这些发现表明，伴有细胞凋亡的突起是在融合的假定区域自主启动的，而不与相对的次级腭部接触。
Epithelial fusion is critical in palatogenesis, and incomplete fusion results in various type of facial cleft, depending on the region that fails to fuse. In mammalian palatogenesis, the bilateral secondary palatal processes fuse in the middle of the face to form the secondary palate. Later, the dorsal side of the secondary palatal shelves fuses with the nasal septum to complete palatogenesis. Importantly, the anterior border of the secondary palatal shelf fuses with the primary palate, which is located at the anterior and ventral border of the nasal septum. While numerous studies have investigated the mechanism of fusion between secondary palatal shelves, very little is known about how the primary palate touches and fuses with the secondary palatal shelves. In this study, we investigate the possible epithelial cell behaviors on the surface of the primary palate using palatal explant cultures of K14-GFP mice. A time-lapse observation of the GFP-labeled epithelium and an SEM analysis revealed that the extrusion epithelium appeared at the region corresponding to the fusing area and expanded rostrally on the nasal septum surface in the absence of the secondary palatal processes. Unlike on the secondary palate surface, cellular migration and subsequent autonomous mesenchymal exposure were not evident on the nasal septum or the primary palate. TUNEL staining revealed that these extrusion epithelia were undergoing apoptosis. These findings indicated that extrusion with apoptosis was autonomously initiated at the presumptive region of the fusion without contact with the opposing secondary palate.
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影响因子：
4.6
作者：
Cuervo, R;Covarrubias, L
通讯作者：
Covarrubias, L
影响因子：
2.7
作者：
Charoenchaikorn, Kesinee;Yokomizo, Tomomasa;Yamashiro, Takashi
通讯作者：
Yamashiro, Takashi
影响因子：
2.7
作者：
Lane, Jamie;Yumoto, Kenji;Azhar, Mohamad;Ninomiya-Tsuji, Jun;Inagaki, Maiko;Hu, Yingling;Deng, Chu-Xia;Kim, Jieun;Mishina, Yuji;Kaartinen, Vesa
通讯作者：
Kaartinen, Vesa
影响因子：
4
作者：
Yu K;Deng M;Naluai-Cecchini T;Glass IA;Cox TC
通讯作者：
Cox TC
影响因子：
4.6
作者：
Sarper SE;Kurosaka H;Inubushi T;Ono Minagi H;Kuremoto KI;Sakai T;Taniuchi I;Yamashiro T
通讯作者：
Yamashiro T