Identification and characterization of genes that control fat deposition in chickens.
Identification and characterization of genes that control fat deposition in chickens.
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DOI：
10.1186/2049-1891-4-43
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发表时间：
2013-11-09
影响因子：
7
通讯作者：
Zhang X
中科院分区：
文献类型：
--
作者：
Claire D'Andre H;Paul W;Shen X;Jia X;Zhang R;Sun L;Zhang X
鸡体内的脂肪沉积对肉质属性，如多汁性、风味、口感和其他感官特性有重要影响。快速生长的鸡的脂肪沉积量比缓慢生长的鸡增加得更快、更早。本研究使用Affymetrix Genechip®鸡基因组阵列32773转录本比较了8周龄快速生长和慢生长鸡肝脏和下丘脑组织的基因表达谱。实时RT-PCR用于验证从微阵列分析中选择的基因的差异表达。在脂肪组织中进一步检测这些基因的mRNA表达。在一个F2资源群体中，研究了4个脂质相关基因的单核苷酸多态性与脂肪性状的关系。在快速生长的鸡和缓慢生长的鸡中，分别鉴定出肝脏组织中的400个基因和下丘脑组织中的220个基因存在差异表达。脂质代谢基因（SULT1B1、ACSBG2、PNPLA3、LPL、AOAH）碳水化合物代谢基因（MGAT4B、XYLB、GBE1、PGM1、HKDC1）胆固醇生物合成基因（FDPS、LSS、HMGCR、NSDHL、DHCR24、IDI1、ME1） HSD17B7等反应或过程基因（CYP1A4、CYP1A1、AKR1B1、CYP4V2、DDO）在速生白隐性岩鸡中表达水平高于生长缓慢的杏化鸡。另一方面，与多细胞生物发育、免疫应答、DNA整合、黑色素生物合成过程、肌肉器官发育和氧化还原相关的基因（FRZB、DMD、FUT8、CYP2C45、DHRSX和CYP2C18）和与糖代谢相关的基因（GCNT2、ELOVL 6和FASN）在高h鸡中的表达水平高于速生鸡。RT-PCR证实了脂肪组织中12个基因中的9个高表达水平。ACSBG2基因的G1257069A和T1247123C与腹部脂肪重量显著相关。FASN基因G4928024A与脂肪带宽、腹脂率显著相关。FASN基因的C4930169T与腹部脂肪重量相关，ELOVL 6基因的A59539099G与皮下脂肪显著相关。滴滴涕的A8378815G与脂肪带宽度相关。快、慢生长鸡脂肪沉积的差异表现为基因表达的差异。
Fat deposits in chickens contribute significantly to meat quality attributes such as juiciness, flavor, taste and other organoleptic properties. The quantity of fat deposited increases faster and earlier in the fast-growing chickens than in slow-growing chickens. In this study, Affymetrix Genechip® Chicken Genome Arrays 32773 transcripts were used to compare gene expression profiles in liver and hypothalamus tissues of fast-growing and slow-growing chicken at 8 wk of age. Real-time RT-PCR was used to validate the differential expression of genes selected from the microarray analysis. The mRNA expression of the genes was further examined in fat tissues. The association of single nucleotide polymorphisms of four lipid-related genes with fat traits was examined in a F2 resource population. Four hundred genes in the liver tissues and 220 genes hypothalamus tissues, respectively, were identified to be differentially expressed in fast-growing chickens and slow-growing chickens. Expression levels of genes for lipid metabolism (SULT1B1, ACSBG2, PNPLA3, LPL, AOAH) carbohydrate metabolism (MGAT4B, XYLB, GBE1, PGM1, HKDC1)cholesttrol biosynthesis (FDPS, LSS, HMGCR, NSDHL, DHCR24, IDI1, ME1) HSD17B7 and other reaction or processes (CYP1A4, CYP1A1, AKR1B1, CYP4V2, DDO) were higher in the fast-growing White Recessive Rock chickens than in the slow-growing Xinghua chickens. On the other hand, expression levels of genes associated with multicellular organism development, immune response, DNA integration, melanin biosynthetic process, muscle organ development and oxidation-reduction (FRZB, DMD, FUT8, CYP2C45, DHRSX, and CYP2C18) and with glycol-metabolism (GCNT2, ELOVL 6, and FASN), were higher in the XH chickens than in the fast-growing chickens. RT-PCR validated high expression levels of nine out of 12 genes in fat tissues. The G1257069A and T1247123C of the ACSBG2 gene were significantly associated with abdominal fat weight. The G4928024A of the FASN gene were significantly associated with fat bandwidth, and abdominal fat percentage. The C4930169T of the FASN gene was associated with abdominal fat weight while the A59539099G of the ELOVL 6 was significantly associated with subcutaneous fat. The A8378815G of the DDT was associated with fat band width. The differences in fat deposition were reflected with differential gene expressions in fast and slow growing chickens.
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人脂肪外植体中时钟基因的昼夜节律。
影响因子：
6.9
作者：
Gomez-Santos, Cecilia;Gomez-Abellan, Purificacion;Madrid, Juan A.;Hernandez-Morante, Juan J.;Lujan, Juan A.;Ordovas, Jose M.;Garaulet, Marta
通讯作者：
Garaulet, Marta
影响因子：
15.9
作者：
FRIED, SK;RUSSELL, CD;BROLIN, RE
通讯作者：
BROLIN, RE
DOI：
10.1016/j.cbpb.2009.02.012
发表时间：
2009-06-01
影响因子：
2.2
作者：
Collin, A.;Swennen, Q.;Tesseraud, S.
通讯作者：
Tesseraud, S.
影响因子：
4.4
作者：
Dunnington, EA;Siegel, PB
通讯作者：
Siegel, PB
影响因子：
4.2
作者：
Jump, DB;Botolin, D;Demeure, O
通讯作者：
Demeure, O