R-SNARE homolog MoSec22 is required for conidiogenesis, cell wall integrity, and pathogenesis of Magnaporthe oryzae.
R-SNARE homolog MoSec22 is required for conidiogenesis, cell wall integrity, and pathogenesis of Magnaporthe oryzae.
复制标题
DOI：
10.1371/journal.pone.0013193
复制
发表时间：
2010-10-06
期刊：
影响因子：
3.7
通讯作者：
Zheng X
中科院分区：
文献类型：
--
作者：
Song W;Dou X;Qi Z;Wang Q;Zhang X;Zhang H;Guo M;Dong S;Zhang Z;Wang P;Zheng X
可溶性N-乙基马来酰亚胺敏感因子附着蛋白受体（SNARE）蛋白介导胞内囊泡融合，这是真核细胞的一个重要细胞过程。为了研究SNARE蛋白在稻瘟病菌中的作用，鉴定了酿酒酵母SNARE蛋白Sec 22的直系同源物MoSec 22，并破坏了MoSEC 22基因。MoSec 22恢复了S.酿酒酵母sec 22突变体对细胞壁扰动剂的抗性较弱，而Δ Mosec 22突变体在菌丝生长、产孢和侵染寄主方面也表现出缺陷。用氧化应激诱导剂处理表明细胞壁完整性的破坏，并且染色和定量测定表明Δ Mosec 22突变体的菌丝侧壁上的异常几丁质沉积。此外，对氧化应激的超敏反应与胞外酶过氧化物酶和漆酶的表达减少相关。因此，我们的研究为Sec 22在真菌生物中的保守功能提供了新的证据，并表明MoSec 22在维持细胞壁完整性方面发挥作用，影响M的生长，形态发生和毒力。米。
Soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) proteins mediate intracellular vesicle fusion, which is an essential cellular process of the eukaryotic cells. To investigate the role of SNARE proteins in the rice blast fungus Magnaporthe oryzae, MoSec22, an ortholog of Saccharomyces cerevisiae SNARE protein Sec22, was identified and the MoSEC22 gene disrupted. MoSec22 restored a S. cerevisiae sec22 mutant in resistance to cell wall perturbing agents, and the ΔMosec22 mutant also exhibited defects in mycelial growth, conidial production, and infection of the host plant. Treatment with oxidative stress inducers indicated a breach in cell wall integrity, and staining and quantification assays suggested abnormal chitin deposition on the lateral walls of hyphae of the ΔMosec22 mutant. Furthermore, hypersensitivity to the oxidative stress correlates with the reduced expression of the extracellular enzymes peroxidases and laccases. Our study thus provides new evidence on the conserved function of Sec22 among fungal organisms and indicates that MoSec22 has a role in maintaining cell wall integrity affecting the growth, morphogenesis, and virulence of M. oryzae.
登录
查看更多内容
影响因子：
7.4
作者：
APOSTOL, I;HEINSTEIN, PF;LOW, PS
通讯作者：
LOW, PS
影响因子：
2.6
作者：
GIETZ, RD;SCHIESTL, RH;WOODS, RA
通讯作者：
WOODS, RA
DOI：
10.1083/jcb.149.1.55
发表时间：
2000-04-03
期刊：
The Journal of cell biology
影响因子：
--
作者：
Cao X;Barlowe C
通讯作者：
Barlowe C
影响因子：
64.8
作者：
Gilbert, MJ;Thornton, CR;Talbot, NJ
通讯作者：
Talbot, NJ
影响因子：
3.5
作者：
Guo, Min;Guo, Wang;Zheng, Xiaobo
通讯作者：
Zheng, Xiaobo