Advances in Manufacturing Cardiomyocytes from Human Pluripotent Stem Cells.
Advances in Manufacturing Cardiomyocytes from Human Pluripotent Stem Cells.
复制标题
从人类多能干细胞制备心肌细胞的研究进展。
DOI：
10.1146/annurev-chembioeng-092120-033922
复制
发表时间：
2022-06-10
影响因子：
8.4
通讯作者：
Palecek, Sean P.
中科院分区：
文献类型：
--
作者：
Floy, Martha E.;Shabnam, Fathima;Simmons, Aaron D.;Bhute, Vijesh J.;Jin, Gyuhyung;Friedrich, Will A.;Steinberg, Alexandra B.;Palecek, Sean P.
关键词：
在过去二十年中，人多能干细胞（hPSC）技术的出现为各种体外和体内应用提供了正常和患病人细胞的来源。值得注意的是，hPSC衍生的心肌细胞（hPSC-CM）被广泛用于模拟人类心脏发育和疾病，并用于治疗心脏病的临床试验。hPSC-CM在这些应用中的成功需要稳健的、可扩展的方法来制造大量安全和有效的细胞。虽然在过去十年中在提高hPSC-CM的纯度和产率以及将分化过程从2D扩展到3D方面取得了重大进展，但在制造过程中诱导成熟表型的努力进展缓慢。过程监控和闭环制造策略正在开发中。在这篇综述中，我们讨论了最近的进展，在hPSC-CM制造，包括分化过程的开发和规模，以及下游工艺，包括分离和稳定。
The emergence of human pluripotent stem cell (hPSC) technology over the past two decades has provided a source of normal and diseased human cells for a wide variety of in vitro and in vivo applications. Notably, hPSC-derived cardiomyocytes (hPSC-CMs) are widely used to model human heart development and disease and are in clinical trials for treating heart disease. Success of hPSC-CMs in these applications requires robust, scalable approaches to manufacture large numbers of safe and potent cells. While significant advances have been made over the past decade in improving the purity and yield of hPSC-CMs and scaling the differentiation process from 2D to 3D, efforts to induce maturation phenotypes during manufacturing have been slow. Process monitoring and closed-loop manufacturing strategies are just being developed. In this review we discuss recent advances in hPSC-CM manufacturing including differentiation process development and scaling, and downstream processes including separation and stabilization.
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影响因子：
4.8
作者：
Correia, Claudia;Serra, Margarida;Espinha, Nuno;Sousa, Marcos;Brito, Catarina;Burkert, Karsten;Zheng, Yunjie;Hescheler, Juergen;Carrondo, Manuel J. T.;Saric, Tomo;Alves, Paula M.
通讯作者：
Alves, Paula M.
影响因子：
48
作者：
Burridge, Paul W.;Matsa, Elena;Shukla, Praveen;Lin, Ziliang C.;Churko, Jared M.;Ebert, Antje D.;Lan, Feng;Diecke, Sebastian;Huber, Bruno;Mordwinkin, Nicholas M.;Plews, Jordan R.;Abilez, Oscar J.;Cui, Bianxiao;Gold, Joseph D.;Wu, Joseph C.
通讯作者：
Wu, Joseph C.
DOI：
10.1007/7651_2020_288
发表时间：
2021-01-01
期刊：
STEM CELLS AND GOOD MANUFACTURING PRACTICES, 2 EDITION
影响因子：
--
作者：
Dashtban, Mehdi;Panchalingam, Krishna Morgan;Baghbaderani, Behnam Ahmadian
通讯作者：
Baghbaderani, Behnam Ahmadian
影响因子：
6.6
作者：
Bhute, Vijesh J.;Bao, Xiaoping;Palecek, Sean P.
通讯作者：
Palecek, Sean P.
DOI：
10.1126/science.1232877
发表时间：
2013-05-10
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Bressan M;Liu G;Mikawa T
通讯作者：
Mikawa T