Peptide alarmone signalling triggers an auto-active bacteriocin necessary for genetic competence.
Peptide alarmone signalling triggers an auto-active bacteriocin necessary for genetic competence.
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DOI：
10.1111/j.1365-2958.2009.06693.x
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发表时间：
2009-05
影响因子：
3.6
通讯作者：
Lévesque CM
中科院分区：
文献类型：
--
作者：
Perry JA;Jones MB;Peterson SN;Cvitkovitch DG;Lévesque CM
遗传能力的诱导是细菌在压力环境条件下增加其遗传库的策略。最近，肺炎链球菌已被证明通过增加负责感受态诱导的肽信息素的表达来协调转化DNA与杀兄弟剂的摄取。在这里，我们的文件，环境压力诱导的表达的肽信息素能力刺激肽（CSP）在口腔病原体变形链球菌。我们发现CSP参与了应激反应，并确定了CSP诱导的调节子在S。通过微阵列分析的变异。与肺炎球菌相反，S.变异株仅在一小部分群体中通过细胞裂解对增加的CSP浓度作出响应。我们专注于细胞裂解的机制，并确定了一种新的细菌素作为“死亡效应”。最重要的是，我们发现这种细菌素通过一种新的作用机制导致细胞死亡：细胞内对抗自我的作用。我们还确定了同源细菌素免疫蛋白，它位于一个单独的非连锁遗传位点，允许其差异调节。裂解反应在S.还讨论了变形能力。总之，这些发现揭示了一种新的自溶途径在S。可能参与口腔生物膜中健身增强基因的传播的变形菌。
The induction of genetic competence is a strategy used by bacteria to increase their genetic repertoire under stressful environmental conditions. Recently, Streptococcus pneumoniae has been shown to co-ordinate the uptake of transforming DNA with fratricide via increased expression of the peptide pheromone responsible for competence induction. Here, we document that environmental stress-induced expression of the peptide pheromone competence-stimulating peptide (CSP) in the oral pathogen Streptococcus mutans. We showed that CSP is involved in the stress response and determined the CSP-induced regulon in S. mutans by microarray analysis. Contrary to pneumococcus, S. mutans responds to increased concentrations of CSP by cell lysis in only a fraction of the population. We have focused on the mechanism of cell lysis and have identified a novel bacteriocin as the ‘death effector’. Most importantly, we showed that this bacteriocin causes cell death via a novel mechanism of action: intracellular action against self. We have also identified the cognate bacteriocin immunity protein, which resides in a separate unlinked genetic locus to allow its differential regulation. The role of the lytic response in S. mutans competence is also discussed. Together, these findings reveal a novel autolytic pathway in S. mutans which may be involved in the dissemination of fitness-enhancing genes in the oral biofilm.
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影响因子：
3.6
作者：
Håvarstein, LS;Martin, B;Claverys, JP
通讯作者：
Claverys, JP
影响因子：
3.2
作者：
Fontaine, Laetitia;Boutry, Celine;Hols, Pascal
通讯作者：
Hols, Pascal
影响因子：
2.1
作者：
Allan, Elaine;Hussain, Haitham A.;Hosie, Arthur H. F.
通讯作者：
Hosie, Arthur H. F.
影响因子：
2.1
作者：
Aspiras, MB;Ellen, RP;Cvitkovitch, DG
通讯作者：
Cvitkovitch, DG
影响因子：
4.4
作者：
Hale, JDF;Ting, YT;Heng, NCK
通讯作者：
Heng, NCK