Magnetic ion channel activation of TREK1 in human mesenchymal stem cells using nanoparticles promotes osteogenesis in surrounding cells.
Magnetic ion channel activation of TREK1 in human mesenchymal stem cells using nanoparticles promotes osteogenesis in surrounding cells.
复制标题
DOI：
10.1177/2041731418808695
复制
发表时间：
2018-01
影响因子：
8.2
通讯作者：
El Haj AJ
中科院分区：
文献类型：
--
作者：
Henstock JR;Rotherham M;El Haj AJ
磁性离子通道激活技术使用与靶向抗体缀合的超顺磁性纳米颗粒直接向细胞表面上的拉伸激活的离子通道施加机械力，刺激机械转导和下游过程。据报道，这种技术可以促进肌肉骨骼细胞类型的分化并增强矿化。以前的研究已经表明，间充质干细胞如何注入到预矿化的环境中，如胎鸡骨骺，导致目标部位的大规模成骨。然而，干细胞和周围宿主组织的相对贡献尚未得到解决，也就是说，是间充质干细胞单独负责观察到的矿化或机械刺激的间充质干细胞也促进宿主组织矿化反应？为了解决这个问题，我们建立了一个新的二维共培养试验，这表明，磁性离子通道激活刺激的人骨髓间充质干细胞不会显着促进迁移，但确实增强周围细胞中的胶原蛋白沉积和矿化。我们得出结论，注射的人间充质干细胞的重要功能之一是释放生物因子（例如，细胞因子和微泡），其引导周围组织反应，并且使用磁性离子通道激活技术远程控制该信号传导过程可能是驱动和调节组织再生和愈合的有用方式。
Magnetic ion channel activation technology uses superparamagnetic nanoparticles conjugated with targeting antibodies to apply mechanical force directly to stretch-activated ion channels on the cell surface, stimulating mechanotransduction and downstream processes. This technique has been reported to promote differentiation towards musculoskeletal cell types and enhance mineralisation. Previous studies have shown how mesenchymal stem cells injected into a pre-mineralised environment such as a foetal chick epiphysis, results in large-scale osteogenesis at the target site. However, the relative contributions of stem cells and surrounding host tissue has not been resolved, that is, are the mesenchymal stem cells solely responsible for the observed mineralisation or do mechanically stimulated mesenchymal stem cells also promote a host-tissue mineralisation response? To address this, we established a novel two-dimensional co-culture assay, which indicated that magnetic ion channel activation stimulation of human mesenchymal stem cells does not significantly promote migration but does enhance collagen deposition and mineralisation in the surrounding cells. We conclude that one of the important functions of injected human mesenchymal stem cells is to release biological factors (e.g., cytokines and microvesicles) which guide the surrounding tissue response, and that remote control of this signalling process using magnetic ion channel activation technology may be a useful way to both drive and regulate tissue regeneration and healing.
登录
查看更多内容
DOI：
10.1016/j.nano.2017.09.008
发表时间：
2018-01-01
影响因子：
5.4
作者：
Rotherham, Michael;Henstock, James R.;El Haj, Alicia J.
通讯作者：
El Haj, Alicia J.
影响因子：
5.2
作者：
Lopez Ponte, Adriana;Marais, Emeline;Domenech, Jorge
通讯作者：
Domenech, Jorge
DOI：
10.1126/stke.2002.119.pe6
发表时间：
2002-02-12
期刊：
Science's STKE : signal transduction knowledge environment
影响因子：
--
作者：
Alenghat, Francis J;Ingber, Donald E
通讯作者：
Ingber, Donald E
影响因子：
3.7
作者：
Linero I;Chaparro O
通讯作者：
Chaparro O
影响因子：
4.5
作者：
Kawai, Takamasa;Katagiri, Wataru;Ueda, Minoru
通讯作者：
Ueda, Minoru