In Vivo Two-Photon Laser Scanning Microscopy with Concurrent Plasma-Mediated Ablation Principles and Hardware Realization
In Vivo Two-Photon Laser Scanning Microscopy with Concurrent Plasma-Mediated Ablation Principles and Hardware Realization
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具有并行等离子体介导消融原理和硬件实现的体内双光子激光扫描显微镜
DOI：
10.1201/9781420076851.ch3
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发表时间：
2009
影响因子：
--
通讯作者：
D. Kleinfeld
中科院分区：
文献类型：
--
作者：
P. Tsai;D. Kleinfeld
许多当前感兴趣的生物过程发生在可接触组织的表层以下。通常情况下，在不影响深层生理和功能的情况下，不容易去除表层。各种成像技术已经被开发出来，使用光学、电和磁造影剂和记录方法进行深入表面的切片。双光子激光扫描显微镜（TPLSM）使用超短（即约100-fs）脉冲激光提供高散射脑组织表面以下500微米或更深深度的光学切片图像[1-5]。该方法的独特之处在于它可以在毫秒时间尺度[6]上提供亚微米级横向分辨率和微米级轴向分辨率的图像，这是研究许多动态生物过程所需要的[1,7 - 11]。
Many biological processes of current interest occur below the surface layers of accessible tissue. It is often the case that the surface layers cannot easily be removed without adversely affecting the physiology and function of the deeper layers. A variety of imaging techniques have been developed to perform sectioning deep to the surface using optical, electrical, and magnetic contrast agents and recording methods. Two-photon laser scanning microscopy (TPLSM) with ultrashort (i.e., order 100-fs) pulsed laser light provides optically sectioned images from depths of 500 microns or more below the surface in highly scattering brain tissue [1–5]. This method is unique in that it can provide images with submicrometer lateral resolution and micrometer axial resolution on the millisecond time-scale [6], as is required for the study of many dynamic biological processes [1,7–11].
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影响因子：
3.4
作者：
Fink, C;Morgan, F;Loew, LM
通讯作者：
Loew, LM
影响因子：
4.8
作者：
Nikolenko, V;Nemet, B;Yuste, R
通讯作者：
Yuste, R
影响因子：
3.4
作者：
Patterson, GH;Piston, DW
通讯作者：
Piston, DW
影响因子：
56.9
作者：
DENK, W;STRICKLER, JH;WEBB, WW
通讯作者：
WEBB, WW
影响因子：
6.1
作者：
Zeng, Fei;Rohde, Christopher B.;Yanik, Mehmet Fatih
通讯作者：
Yanik, Mehmet Fatih