Pathogen-induced biosynthetic pathways encode defense-related molecules in bread wheat.
Pathogen-induced biosynthetic pathways encode defense-related molecules in bread wheat.
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DOI：
10.1073/pnas.2123299119
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发表时间：
2022-04-19
影响因子：
11.1
通讯作者：
中科院分区：
文献类型：
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作者：
小麦是全球重要的粮食作物，由于严重疾病的爆发而遭受重大产量损失。更好地理解小麦对病原体攻击的反应机制可以确定提高抗病性的新策略。在这里，我们发现了六种病原体诱导的生物合成途径，它们共享一个共同的调控网络，并形成了精心策划的防御反应的一部分。对小麦基因组的研究表明，这些途径均由生物合成基因簇（BGC）编码。我们进一步表明，这些BGC产生类黄酮和萜烯，可能作为植物抗毒素或防御相关的信号分子。我们的研究结果为小麦生物胁迫反应的分子基础提供了关键的见解，并为作物改良开辟了潜在的途径。小麦是一种广泛种植的粮食作物，由于害虫和病原体的侵袭而遭受重大产量损失。因此，更好地了解小麦的生物胁迫反应是非常重要的。最近组装的面包小麦基因组加上广泛的转录组学资源提供了前所未有的新机会，调查响应病原体的挑战。在这里，我们分析基因共表达网络，以确定模块显示一致的诱导响应病原体暴露。在顶级病原体诱导的模块，我们确定了多个集群的物理相邻的基因，对应于六个病原体诱导的生物合成途径，共享一个共同的监管网络。功能分析表明，这些途径，所有这些都是由生物合成基因簇编码，产生各种不同类别的化合物，即类黄酮，二萜，三萜，包括防御相关的化合物埃拉霉素。通过比较基因组学，我们还确定了与已知的水稻植物抗毒素momilactones，以及与防御相关的基因簇在草模式植物二穗短柄草。我们的研究结果大大推进了对小麦化学防御的理解，并为增强这一农业重要作物的抗病性开辟了途径。他们还证实了转录网络的力量，发现了具有大型复杂基因组的植物中化学防御的生物合成。
Wheat is a globally important food crop that suffers major yield losses due to outbreaks of severe disease. A better mechanistic understanding of how wheat responds to pathogen attack could identify new strategies for enhancing disease resistance. Here, we discover six pathogen-induced biosynthetic pathways that share a common regulatory network and form part of an orchestrated defense response. Investigation of the wheat genome reveals that these pathways are each encoded by biosynthetic gene clusters (BGCs). We further show that these BGCs produce flavonoids and terpenes that may serve as phytoalexins or defense-related signaling molecules. Our results provide key insights into the molecular basis of biotic stress responses in wheat and open potential avenues for crop improvement. Wheat is a widely grown food crop that suffers major yield losses due to attack by pests and pathogens. A better understanding of biotic stress responses in wheat is thus of major importance. The recently assembled bread wheat genome coupled with extensive transcriptomic resources provides unprecedented new opportunities to investigate responses to pathogen challenge. Here, we analyze gene coexpression networks to identify modules showing consistent induction in response to pathogen exposure. Within the top pathogen-induced modules, we identify multiple clusters of physically adjacent genes that correspond to six pathogen-induced biosynthetic pathways that share a common regulatory network. Functional analysis reveals that these pathways, all of which are encoded by biosynthetic gene clusters, produce various different classes of compounds—namely, flavonoids, diterpenes, and triterpenes, including the defense-related compound ellarinacin. Through comparative genomics, we also identify associations with the known rice phytoalexins momilactones, as well as with a defense-related gene cluster in the grass model plant Brachypodium distachyon. Our results significantly advance the understanding of chemical defenses in wheat and open up avenues for enhancing disease resistance in this agriculturally important crop. They also exemplify the power of transcriptional networks to discover the biosynthesis of chemical defenses in plants with large, complex genomes.
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影响因子：
4.4
作者：
Dobon A;Bunting DC;Cabrera-Quio LE;Uauy C;Saunders DG
通讯作者：
Saunders DG
影响因子：
3.5
作者：
Hasegawa, Morifumi;Mitsuhara, Ichiro;Ohashi, Yuko
通讯作者：
Ohashi, Yuko
影响因子：
56.9
作者：
Frey, M;Chomet, P;Gierl, A
通讯作者：
Gierl, A
影响因子：
16.6
作者：
Li Y;Leveau A;Zhao Q;Feng Q;Lu H;Miao J;Xue Z;Martin AC;Wegel E;Wang J;Orme A;Rey MD;Karafiátová M;Vrána J;Steuernagel B;Joynson R;Owen C;Reed J;Louveau T;Stephenson MJ;Zhang L;Huang X;Huang T;Fan D;Zhou C;Tian Q;Li W;Lu Y;Chen J;Zhao Y;Lu Y;Zhu C;Liu Z;Polturak G;Casson R;Hill L;Moore G;Melton R;Hall N;Wulff BBH;Doležel J;Langdon T;Han B;Osbourn A
通讯作者：
Osbourn A
DOI：
10.1111/tpj.13249
发表时间：
2016-10
期刊：
The Plant journal : for cell and molecular biology
影响因子：
--
作者：
Kitaoka N;Wu Y;Zi J;Peters RJ
通讯作者：
Peters RJ