Nitrogen loss through anaerobic ammonium oxidation in agricultural drainage ditches
Nitrogen loss through anaerobic ammonium oxidation in agricultural drainage ditches
复制标题
农业排水沟厌氧氨氧化氮损失
DOI：
10.1007/s00374-015-1058-4
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发表时间：
2015-09
影响因子：
6.5
通讯作者：
Ma Shi-jie
中科院分区：
文献类型：
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作者：
Shen Li-dong;Zheng Pei-hui;Ma Shi-jie
农业排水沟是氮从农田到地表水的重要管道。反硝化已被证明是农业排水沟渠重要的脱氮途径；然而，目前尚不清楚厌氧氨氧化（anammox）的作用。在这里，我们调查了位于中国江苏省南京市的六个不同农业排水沟中厌氧氨氧化造成的氮损失。稳定同位素标记实验证实了每条检查沟渠中的厌氧氨氧化活性，潜在的厌氧氨氧化率范围为 4.4 至 23.8 nmol N2g−1 沉积物 day−1。厌氧氨氧化过程对沉积物二氮气体的产生贡献了 2.1-18.8%，其余部分则来自反硝化作用。据估计，每年 6.1–32.9 g N m-2 的总损失可归因于所检查沟渠中的厌氧氨氧化过程。基于 Illumina 的 16S rRNA 基因测序证实了所检查的沉积物中存在多种厌氧氨氧化细菌，包括 Candidatus Brocadia、Candidatus Kuenenia 和 Candidatus Anamoxoglobus。定量PCR进一步证实了厌氧氨氧化细菌的存在，其数量在1.9 × 104和1.2 × 105copies g−1沉积物之间变化。我们的研究结果显示了农业排水沟中厌氧氨氧化过程的证据，并证明了厌氧氨氧化对农业排水沟脱氮的贡献可能不可忽视。
Agricultural drainage ditches are a key conduit of nitrogen from agricultural fields to surface water. Denitrification has been proven to be an important N removal pathway in agricultural drainage ditches; however, it is currently unknown about the role of anaerobic ammonium oxidation (anammox). Here, we investigated the N loss through anammox in six different agricultural drainage ditches, located in Nanjing City, Jiangsu Province (China). Stable isotope-labeling experiments confirmed the anammox activity in every examined ditch, and the potential anammox rates ranged from 4.4 to 23.8 nmol N2g−1sediment day−1. The anammox process contributed 2.1–18.8 % to sediment dinitrogen gas production, while the remainder would be due to denitrification. It is estimated that a total loss of 6.1–32.9 g N m-2per year could be attributed to the anammox process in the examined ditches. Illumina-based 16S rRNA gene sequencing confirmed the presence of diverse anammox bacteria in the examined sediments, includingCandidatusBrocadia,CandidatusKuenenia, andCandidatusAnammoxoglobus. Quantitative PCR further confirmed the presence of anammox bacteria, varying between 1.9 × 104and 1.2 × 105copies g−1sediment. The results of our study showed the evidence of anammox process in agricultural drainage ditches and demonstrated that the contribution of anammox to N removal from agricultural drainage ditches may not be negligible.
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水产养殖池塘淡水沉积物中厌氧氨氧化过程的证据
影响因子：
5.8
作者：
Shen Li-dong;Wu Hong-sheng;Gao Zhi-qiu;Ruan Yun-jie;Xu Xiang-hua;Li Ji;Ma Shi-jie;Zheng Pei-hui
通讯作者：
Zheng Pei-hui
影响因子：
5.1
作者：
D. Woebken;P. Lam;M. Kuypers;S. Naqvi;B. Kartal;M. Strous;M. Jetten;B. Fuchs;R. Amann
通讯作者：
D. Woebken;P. Lam;M. Kuypers;S. Naqvi;B. Kartal;M. Strous;M. Jetten;B. Fuchs;R. Amann
DOI：
10.1128/genomea.00267-15
发表时间：
2015-04-16
期刊：
Genome announcements
影响因子：
--
作者：
Oshiki M;Shinyako-Hata K;Satoh H;Okabe S
通讯作者：
Okabe S
DOI：
10.1080/10934520500184590
发表时间：
2005-10
期刊：
Journal of Environmental Science and Health, Part A
影响因子：
--
作者：
Ai-Jie Wang;Da-zhong Du;N. Ren;J. V. van Groenestijn
通讯作者：
Ai-Jie Wang;Da-zhong Du;N. Ren;J. V. van Groenestijn
影响因子：
3.8
作者：
S. Ullah;S. Faulkner
通讯作者：
S. Ullah;S. Faulkner