Evidence for anaerobic ammonium oxidation process in freshwater sediments of aquaculture ponds
Evidence for anaerobic ammonium oxidation process in freshwater sediments of aquaculture ponds
复制标题
水产养殖池塘淡水沉积物中厌氧氨氧化过程的证据
DOI：
10.1007/s11356-015-5356-z
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发表时间：
2015
影响因子：
5.8
通讯作者：
Zheng Pei-hui
中科院分区：
文献类型：
--
作者：
Shen Li-dong;Wu Hong-sheng;Gao Zhi-qiu;Ruan Yun-jie;Xu Xiang-hua;Li Ji;Ma Shi-jie;Zheng Pei-hui
厌氧氨氧化(Anammox)工艺可以同时去除对水生动物有毒的氨氮和亚硝酸盐，对水产养殖业非常重要。采用基于Illumina的16S rRNA基因测序、定量聚合酶链式反应和15N稳定同位素测定等方法，对4个不同淡水养殖池塘底泥中厌氧氨氧化细菌的存在和活性进行了研究。在所研究的池塘底泥中检出不同属的厌氨氧化细菌，包括假丝酵母菌属(Candidatus Brocadia)、昆氏假丝酵母属(Candidatus Kuenenia)和假丝酵母属(Candidatus Anammoglobus)，其中以假丝酵母菌属(Candidatus Brocadia)占优势。肼合成酶基因的定量聚合酶链式反应显示，受试池塘中厌氧氨氧化细菌的丰度为5.6 × 10 4~2.1 × 10 5拷贝g−1。N2g−1沉淀物−1的潜在厌氧氨氧化速率为3.7nmo1~19.4nmoL，N2g−1沉淀物1−1的潜在反硝化速率为107.1~300.3 nmoL。据估计，每年总损失2.1g-10.9g·N/m−2可归因于所研究池塘的厌氨氧化过程，这表明该过程可能有助于淡水养殖池塘的脱氮。
The anaerobic ammonium oxidation (anammox) process, which can simultaneously remove ammonium and nitrite, both toxic to aquatic animals, can be very important to the aquaculture industry. Here, the presence and activity of anammox bacteria in the sediments of four different freshwater aquaculture ponds were investigated by using Illumina-based 16S rRNA gene sequencing, quantitative PCR assays and15N stable isotope measurements. Different genera of anammox bacteria were detected in the examined pond sediments, includingCandidatusBrocadia,CandidatusKuenenia andCandidatusAnammoxoglobus, withCandidatusBrocadia being the dominant anammox genus. Quantitative PCR of hydrazine synthase genes showed that the abundance of anammox bacteria ranged from 5.6 × 104to 2.1 × 105copies g−1sediment in the examined ponds. The potential anammox rates ranged between 3.7 and 19.4 nmol N2g−1sediment day−1, and the potential denitrification rates varied from 107.1 to 300.3 nmol N2g−1sediment day−1. The anammox process contributed 1.2–15.3 % to sediment dinitrogen gas production, while the remainder would be due to denitrification. It is estimated that a total loss of 2.1–10.9 g N m−2per year could be attributed to the anammox process in the examined ponds, suggesting that this process could contribute to nitrogen removal in freshwater aquaculture ponds.
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影响因子：
5.1
作者：
D. Woebken;P. Lam;M. Kuypers;S. Naqvi;B. Kartal;M. Strous;M. Jetten;B. Fuchs;R. Amann
通讯作者：
D. Woebken;P. Lam;M. Kuypers;S. Naqvi;B. Kartal;M. Strous;M. Jetten;B. Fuchs;R. Amann
影响因子：
3.7
作者：
Wu S;Wang G;Angert ER;Wang W;Li W;Zou H
通讯作者：
Zou H
影响因子：
4.5
作者：
M. V. Kessel;H. Harhangi;K. Pas-Schoonen;J. Vossenberg;G. Flik;M. Jetten;P. Klaren;H. J. M. Camp
通讯作者：
M. V. Kessel;H. Harhangi;K. Pas-Schoonen;J. Vossenberg;G. Flik;M. Jetten;P. Klaren;H. J. M. Camp
影响因子：
15.5
作者：
Fadrosh DW;Ma B;Gajer P;Sengamalay N;Ott S;Brotman RM;Ravel J
通讯作者：
Ravel J
影响因子：
1.4
作者：
Risgaard-Petersen, N;Meyer, RL;Revsbech, NP
通讯作者：
Revsbech, NP