Clathrin-mediated endocytosis is a candidate entry sorting mechanism for Bombyx mori cypovirus.
Clathrin-mediated endocytosis is a candidate entry sorting mechanism for Bombyx mori cypovirus.
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网格蛋白介导的内吞作用是家蚕病毒的候选进入分选机制
DOI：
10.1038/s41598-018-25677-1
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发表时间：
2018-05-08
影响因子：
4.6
通讯作者：
Gong C
中科院分区：
文献类型：
--
作者：
Chen F;Zhu L;Zhang Y;Kumar D;Cao G;Hu X;Liang Z;Kuang S;Xue R;Gong C
Bombyx mori cypovirus (BmCPV), a member of the Reoviridae, specifically infects silkworms and causes extensive economic losses to the sericulture industry. To date, the entry mechanism of BmCPV into cells is unclear. Here we used electron microscopy to study the route of entry of BmCPV into cells, and the results demonstrated that the entry of BmCPV into BmN cells was mediated by endocytosis. Blocking the entry pathway with four endocytosis inhibitors, including dansylcadaverine, chlorpromazine, genistein, and PP2, significantly decreased the infectivity of BmCPV. This indicates that BmCPV enters BmN cells via endocytosis, and that clathrin-mediated sorting is the predominant entry method. After the relative expression levels of clathrin heavy chain (clathrin, GenBank accession No. NM_001142971.1) and the adaptor protein complex-1 gamma subunit AP-1 (AP-1, GenBank accession No. JQ824201.1), which are involved in clathrin-mediated endocytosis, were inhibited by RNA interference or abolishing the functions of clathrin and AP-1 with their corresponding antibodies, the infectivity of BmCPV was reduced significantly, which suggests that clathrin-mediated endocytosis contributed to the entry of BmCPV into cells. Our findings suggest that the clathrin-mediated endocytosis pathway is a candidate for the development of therapeutics for silkworm cytoplasmic polyhedrosis.
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影响因子：
5.4
作者：
Diaz-Salinas, Marco A.;Romero, Pedro;Arias, Carlos F.
通讯作者：
Arias, Carlos F.
影响因子：
3.8
作者：
Hagiwara, K;Rao, SJ;Carner, GR
通讯作者：
Carner, GR
DOI：
10.1016/j.bbamcr.2014.12.030
发表时间：
2015-03-01
影响因子：
5.1
作者：
Kibria, K. M. Kaderi;Rawat, Khushboo;Malhotra, Pawan
通讯作者：
Malhotra, Pawan
影响因子：
5.6
作者：
FUJIIKAWATA, I;MIURA, KI;FUKE, M
通讯作者：
FUKE, M
影响因子：
5.4
作者：
Maginnis, MS;Forrest, JC;Dermody, TS
通讯作者：
Dermody, TS