Induced pluripotent stem cells are sensitive to DNA damage.
Induced pluripotent stem cells are sensitive to DNA damage.
复制标题
诱导多能干细胞对 DNA 损伤敏感
DOI：
10.1016/j.gpb.2013.09.006
复制
发表时间：
2013-10
影响因子：
9.5
通讯作者：
Sun, Yingli
中科院分区：
文献类型：
--
作者：
Zhang, Minjie;Yang, Caiyun;Liu, Huixian;Sun, Yingli
Induced pluripotent stem cells (iPSCs) resemble embryonic stem cells (ESCs) in morphology, gene expression and in vitro differentiation, raising new hope for personalized clinical therapy. While many efforts have been made to improve reprogramming efficiency, significant problems such as genomic instability of iPSCs need to be addressed before clinical therapy. In this study, we try to figure out the real genomic state of iPSCs and their DNA damage response to ionizing radiation (IR). We found that iPSC line 3FB4-1 had lower DNA damage repair ability than mouse embryonic fibroblast (MEF) cells, from which 3FB4-1line was derived. After the introduction of DNA damage by IR, the number of γ-H2AX foci in 3FB4-1 increased modestly compared to a large increase seen in MEF, albeit both significantly (P < 0.01). In addition, whole-genome sequencing analysis showed that after IR, 3FB4-1 possessed more point mutations than MEF and the point mutations spread all over chromosomes. These observations provide evidence that iPSCs are more sensitive to ionizing radiation and their relatively low DNA damage repair capacity may account for their high radiosensitivity. The compromised DNA damage repair capacity of iPSCs should be considered when used in clinical therapy.
登录
查看更多内容
影响因子：
30.8
作者：
Khanna, KK;Jackson, SP
通讯作者：
Jackson, SP
影响因子：
12.4
作者：
通讯作者：
--
DOI：
10.1093/bioinformatics/btp352
发表时间：
2009-08-15
期刊：
Bioinformatics (Oxford, England)
影响因子：
--
作者：
Li H;Handsaker B;Wysoker A;Fennell T;Ruan J;Homer N;Marth G;Abecasis G;Durbin R;1000 Genome Project Data Processing Subgroup
通讯作者：
1000 Genome Project Data Processing Subgroup
影响因子：
64.8
作者：
通讯作者：
--
影响因子：
46.9
作者：
Huangfu, Danwei;Maehr, Rene;Melton, Douglas A.
通讯作者：
Melton, Douglas A.