A Hydrogen-Sulfide-Repressed Methionine Synthase SlMS1 Acts as a Positive Regulator for Fruit Ripening in Tomato.
A Hydrogen-Sulfide-Repressed Methionine Synthase SlMS1 Acts as a Positive Regulator for Fruit Ripening in Tomato.
复制标题
DOI：
10.3390/ijms232012239
复制
发表时间：
2022-10-13
影响因子：
5.6
通讯作者：
中科院分区：
文献类型：
--
作者：
乙烯是跃变型果实成熟的关键激素，蛋氨酸是乙烯的间接前体物质。然而，甲硫氨酸合成酶是否在番茄果实成熟中起作用仍然是未知的。在这项研究中，我们发现，番茄甲硫氨酸合酶（命名为SLMS 1），这可能是在转录水平上被抑制的硫化氢（H2S），作为一个积极的调节番茄果实成熟。通过生物信息学分析发现，番茄中的SlMS 1和SlMS 2与拟南芥中的蛋氨酸脱氢酶高度同源。分析了SlMS 1和SlMS 2在番茄中的表达模式，结果表明SlMS 1在果实成熟过程中表达上调，提示其在果实成熟调控中具有潜在的作用。在SlMS 1的氨基酸序列中发现了潜在的二分核定位信号;因此，用GFP标记SlMS 1并在本氏烟草的叶片中观察到。一致地，SlMS 1-GFP显示出强的核定位和细胞质定位。在番茄果实中通过瞬时沉默（病毒诱导的基因沉默，VIGS）和瞬时过表达研究SlMS 1在调节果实成熟中的作用。结果表明，SlMS 1沉默导致果实成熟延迟，表现为更多的叶绿素和更少的类胡萝卜素积累，而SlMS 1过表达与对照相比显著加速果实成熟。进一步研究表明，SlMS 1过表达可上调类胡萝卜素合成相关基因（PSY 1、PDS、ZDS）、叶绿素降解相关基因（NYC 1、PAO、PPH、SGR 1）、细胞壁代谢相关基因（CEL 2、EXP、PG、TBG 4、XTH 5）和乙烯合成途径相关基因（ACO 1、ACO 3、ACS 2）的表达，而SlMS 1沉默则相反。相关性分析表明，SlMS 1基因表达量与叶绿素含量呈负相关，与类胡萝卜素和成熟相关基因表达量呈正相关。综上所述，我们的数据表明，SlMS 1是番茄果实成熟的正调控因子，可能是H2S延迟成熟效应的靶基因。
Ethylene is a key phytohormone that regulates the ripening of climacteric fruits, and methionine is an indirect precursor of ethylene. However, whether methionine synthase plays a role in fruit ripening in Solanum lycopersicum (tomato) is still unknown. In this study, we find that a tomato methionine synthase (named SlMS1), which could be repressed at the transcriptional level by hydrogen sulfide (H2S), acts as a positive regulator for tomato fruit ripening. By a bioinformatics analysis, it is found that SlMS1 and SlMS2 in tomato are highly homologous to methionine synthases in Arabidopsis thaliana. The expression pattern of SlMS1 and SlMS2 is analyzed in tomato, and SlMS1 expression is up-regulated during fruit ripening, suggesting its potential role in regulating fruit ripening. A potential bipartite nuclear localization signal is found in the amino acid sequence of SlMS1; thus, SlMS1 is tagged with GFP and observed in the leaves of Nicotiana benthamiana. Consistently, SlMS1-GFP shows strong nuclear localization and also cytoplasmic localization. The role of SlMS1 in regulating fruit ripening is investigated in tomato fruit by transient silencing (virus-induced gene silencing, VIGS) and transient overexpression. The results show that SlMS1 silencing causes delayed fruit ripening, evidenced by more chlorophyll and less carotenoid accumulation, while SlMS1 overexpression accelerates fruit ripening significantly compared with control. Further investigation shows that SlMS1 overexpression could up-regulate the expression of carotenoid-synthesis-related genes (PSY1, PDS, ZDS), chlorophyll-degradation-related genes (NYC1, PAO, PPH, SGR1), cell-wall-metabolism-related genes (CEL2, EXP, PG, TBG4, XTH5) and ethylene-synthesis-pathway-related genes (ACO1, ACO3, ACS2), while SlMS1 silencing causes the opposite results. The correlation analysis indicates that SlMS1 expression is negatively correlated with chlorophyll content and positively correlated with carotenoid and ripening-related gene expressions. Taken together, our data suggest that SlMS1 is a positive regulator of tomato fruit ripening and a possible target gene for the ripening-delaying effect of H2S.
登录
查看更多内容
影响因子：
--
作者：
通讯作者：
--
影响因子：
5.6
作者：
Huo J;Huang D;Zhang J;Fang H;Wang B;Wang C;Liao W
通讯作者：
Liao W
SlSGRL 是一种番茄 SGR 样蛋白，可促进 ABA 信号通路下游的叶绿素降解
影响因子：
6.5
作者：
Yang, Minmin;Zhu, Shaobo;Lv, Wei
通讯作者：
Lv, Wei
番茄果实中病毒诱导的基因沉默。
影响因子：
7.2
作者：
Fu, DQ;Zhu, BZ;Luo, YB
通讯作者：
Luo, YB
影响因子：
10.7
作者：
Kumar, Sudhir;Stecher, Glen;Tamura, Koichiro
通讯作者：
Tamura, Koichiro