Disruption of the gene for Met-tRNA(fMet) formyltransferase severely impairs growth of Escherichia coli
Disruption of the gene for Met-tRNA(fMet) formyltransferase severely impairs growth of Escherichia coli
复制标题
Met-tRNA（fMet）甲酰基转移酶基因的破坏严重损害大肠杆菌的生长
作者：
J. Guillon;Y. Mechulam;J. Schmitter;S. Blanquet;G. Fayat
In bacteria, as well as in chloroplasts and mitochondria, the free amino group of the methionylated initiator tRNA(fMet) is specifically modified by the addition of a formyl group. The importance of this modification remains unclear. With the availability of pure Escherichia coli 10-formyltetrahydrofolate:L-methionyl-tRNA(fMet) N-formyltransferase, the enzyme catalyzing Met-tRNA(fMet) formylation, the corresponding fmt gene and its flanking regions were cloned and sequenced. The chromosomal fmt gene was disrupted, and strains modified in their formylation activity were constructed. A depletion of the cellular formylation activity was accompanied by a decrease in the growth rate of the bacteria. At 37 degrees C, in a rich medium, the absence of a functional fmt gene reduced the growth rate to 0.28 doubling per h, from 2.3 for the control strain. At 42 degrees C, the studied fmt mutant strain did not grow further.
登录
查看更多内容
起始子 tRNA 的突变体，既充当起始子又充当延伸子。
DOI：
--
发表时间：
1991
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Varshney,U;Lee,CP;Seong,BL;RajBhandary,UL
通讯作者：
RajBhandary,UL
影响因子：
10.5
作者：
HARTZ, D;MCPHEETERS, DS;GOLD, L
通讯作者：
GOLD, L
DOI：
10.1016/s0021-9258(18)47560-6
发表时间：
1987-06
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
D. Ebbole;H. Zalkin
通讯作者：
D. Ebbole;H. Zalkin
大肠杆菌甘氨酰胺核糖核苷酸转化酶的亚克隆、表征和亲和标记。
影响因子：
2.9
作者：
Inglese,J;Johnson,DL;Shiau,A;Smith,JM;Benkovic,SJ
通讯作者：
Benkovic,SJ
通过酵母突变的功能互补克隆三个人类多功能从头嘌呤生物合成基因。
DOI：
10.1073/pnas.87.8.2916
发表时间：
1990
影响因子：
11.1
作者：
Schild,D;Brake,AJ;Kiefer,MC;Young,D;Barr,PJ
通讯作者：
Barr,PJ