Hormonal Regulatory Patterns of LaKNOXs and LaBEL1 Transcription Factors Reveal Their Potential Role in Stem Bulblet Formation in LA Hybrid Lily.
Hormonal Regulatory Patterns of LaKNOXs and LaBEL1 Transcription Factors Reveal Their Potential Role in Stem Bulblet Formation in LA Hybrid Lily.
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LaKNOXs 和 LaBEL1 转录因子的激素调节模式揭示了它们在 LA 杂交百合茎球形成中的潜在作用
DOI：
10.3390/ijms222413502
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发表时间：
2021-12-16
影响因子：
5.6
通讯作者：
Lyu Y
中科院分区：
文献类型：
--
作者：
Zhang Y;Zeng Z;Yong Y;Lyu Y
在百合繁殖中，鳞茎的形成机制一直是研究的热点。然而，茎小鳞茎形成的研究有限。茎小鳞茎形成于地上茎和地下茎的叶腋处，为百合提供了强大的自繁能力。结果表明：100 mg/L 6-BA可诱导LA杂交百合'Aladdin'地上茎段产生小鳞茎，其内源激素IAA和GA_4含量降低，细胞分裂素相对含量增加;利用RT-qPCR技术分析了茎小鳞茎形成过程中3个潜在基因（2个KNOX和1个BELL）的表达模式，结果表明KNOX基因表达量呈下降趋势，BELL基因表达量呈上升趋势。利用RACE技术从L.“阿拉丁”，并表示LaBEL 1。LaKNOX 1-LaBEL 1和LaKNOX 1-LaKNOX 2的物理相互作用通过酵母双杂交和双分子荧光互补试验证实。此外，在转基因拟南芥中还发现了LaKNOX 1、LaKNOX 2和LaBEL 1基因及其异源二聚体的激素调控模式，表明LaKNOX 1、LaKNOX 2和LaBEL 1基因在茎鳞茎形成过程中的大量mRNA积累可能导致细胞分裂素的相对增加和GA和IAA的相对减少。LaKNOX 1与LaKNOX 2和LaBEL 1相互作用，同时调节多种植物激素以维持适当的激素稳态，这表明它们在百合茎小鳞茎形成中具有潜在的作用。阿拉丁
In lily reproduction, the mechanism of formation of bulbs has been a hot topic. However, studies on stem bulblet formation are limited. Stem bulblets, formed in the leaf axils of under- and above-ground stems, provide lilies with a strong capacity for self-propagation. First, we showed that above-ground stem bulblets can be induced by spraying 100 mg/L 6-BA on the LA hybrid lily ‘Aladdin’, with reduced endogenous IAA and GA4 and a higher relative content of cytokinins. Then, expression patterns of three potential genes (two KNOTTED1-like homeobox (KNOX) and one partial BEL1-like homeobox (BELL)), during stem bulblet formation from our previous study, were determined by RT-qPCR, presenting a down-up trend in KNOXs and a rising tendency in BELL. The partial BELL gene was cloned by RACE from L. ‘Aladdin’ and denoted LaBEL1. Physical interactions of LaKNOX1-LaBEL1 and LaKNOX1-LaKNOX2 were confirmed by yeast two-hybrid and bimolecular fluorescence complementation assays. Furthermore, hormonal regulatory patterns of single LaKNOX1, LaKNOX2, LaBEL1, and their heterodimers, were revealed in transgenic Arabidopsis, suggesting that the massive mRNA accumulations of LaKNOX1, LaKNOX2 and LaBEL1 genes during stem bulblet formation could cause the dramatic relative increase of cytokinins and the decline of GAs and IAA. Taken together, a putative model was proposed that LaKNOX1 interacts with LaKNOX2 and LaBEL1 to regulate multiple phytohormones simultaneously for an appropriate hormonal homeostasis, which suggests their potential role in stem bulblet formation in L. ‘Aladdin’.
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影响因子：
14.9
作者：
Cole, M;Nolte, C;Werr, W
通讯作者：
Werr, W
影响因子：
11.6
作者：
Brambilla, Vittoria;Battaglia, Raffaella;Colombo, Lucia
通讯作者：
Colombo, Lucia
影响因子：
7.4
作者：
Chen, H;Rosin, FM;Hannapel, DJ
通讯作者：
Hannapel, DJ
影响因子：
11.6
作者：
KERSTETTER, R;VOLLBRECHT, E;HAKE, S
通讯作者：
HAKE, S
影响因子：
11.6
作者：
Bolduc, Nathalie;Hake, Sarah
通讯作者：
Hake, Sarah