Nuclear import of the transcription factor SHOOT MERISTEMLESS depends on heterodimerization with BLH proteins expressed in discrete sub-domains of the shoot apical meristem of Arabidopsis thaliana.
Nuclear import of the transcription factor SHOOT MERISTEMLESS depends on heterodimerization with BLH proteins expressed in discrete sub-domains of the shoot apical meristem of Arabidopsis thaliana.
复制标题
DOI：
10.1093/nar/gkl016
复制
发表时间：
2006
影响因子：
14.9
通讯作者：
Werr, W
中科院分区：
文献类型：
--
作者：
Cole, M;Nolte, C;Werr, W
基因SHOOT MERISTEMLESS（STM）是拟南芥茎顶端分生组织（SAM）的起始和维持所必需的，并且编码MEINOX/三氨基酸环延伸（TALE）-HD型转录因子。与绿色荧光蛋白的翻译融合表明，STM默认不是核。在酵母双杂交筛选与分生组织富集的cDNA文库，三个相互作用的BLH（Bel 1样同源结构域）转录因子被确定。根据双分子荧光互补，STM通过与BLH伴侣蛋白的异源二聚化被靶向到核隔室中，BLH伴侣蛋白在从中心到外围的不同SAM结构域中表达。在功能水平上，在转基因拟南芥植物中的过表达实验表明，个别异源二聚体提供不同的贡献。这些结果有助于我们理解STM转录因子在SAM中的功能，也为TALE HD超基因家族在动植物谱系中的进化提供了新的线索。
The gene SHOOT MERISTEMLESS (STM) is required for the initiation and the maintenance of the shoot apical meristem (SAM) in Arabidopsis and encodes a MEINOX/three amino acid loop extension (TALE)-HD-type transcription factor. Translational fusions with the green fluorescent protein showed that STM is not nuclear by default. In a yeast two-hybrid screen performed with a meristem-enriched cDNA library, three interacting BLH (Bel1-like homeodomain) transcription factors were identified. According to bimolecular fluorescence complementation, STM is targeted into the nuclear compartment through heterodimerization with BLH partner proteins, which are expressed in distinct SAM domains from the center to the periphery. On a functional level, overexpression experiments in transgenic Arabidopsis plants suggest that individual heterodimers provide distinct contributions. These results contribute to our understanding of the STM transcription factor function in the SAM and also shed new light on the evolution of the TALE-HD super gene family in animal and plant lineages.
登录
查看更多内容
影响因子：
2.4
作者：
Becker, A;Bey, M;Theissen, G
通讯作者：
Theissen, G
影响因子：
2.4
作者：
Burglin, TR
通讯作者：
Burglin, TR
影响因子：
11.4
作者：
Kilstrup-Nielsen, C;Alessio, M;Zappavigna, V
通讯作者：
Zappavigna, V
影响因子：
11.4
作者：
Berthelsen, J;Zappavigna, V;Blasi, F
通讯作者：
Blasi, F
影响因子：
7.4
作者：
Brand, U;Grünewald, M;Simon, R
通讯作者：
Simon, R