Uptake, tissue distribution, and toxicity of polystyrene nanoparticles in developing zebrafish (Danio rerio).
Uptake, tissue distribution, and toxicity of polystyrene nanoparticles in developing zebrafish (Danio rerio).
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DOI：
10.1016/j.aquatox.2017.11.017
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发表时间：
2018-01
期刊：
影响因子：
--
通讯作者：
Di Giulio RT
中科院分区：
文献类型：
--
作者：
Pitt JA;Kozal JS;Jayasundara N;Massarsky A;Trevisan R;Geitner N;Wiesner M;Levin ED;Di Giulio RT
塑料污染是一个严重的环境问题，海洋中的大部分人为垃圾都是塑料污染，包括宏观、微观和可能的纳米级（至少一个维度小于100纳米）塑料颗粒。虽然宏观塑料和微塑料的毒性研究相对较好，但纳米塑料的毒性在很大程度上是未知的。本研究利用荧光聚苯乙烯纳米颗粒（PS NPs）研究了纳米塑料对发育中的斑马鱼（Danio rerio）的潜在毒性，并表征了颗粒在胚胎和幼虫中的摄取和分布。在受精后6小时（hpf）的斑马鱼胚胎暴露于PS NPs(0.1, 1或10 ppm)，直到120 hpf。我们的研究结果表明，PS NPs早在24 hpf时就在卵黄囊中积累，并在整个发育过程中（48 hpf-120 hpf）迁移到胃肠道、胆囊、肝脏、胰腺、心脏和大脑。PS NPs的积累在净化期（120 hpf-168 hpf）在所有器官中都有所减少，但在胰腺和胃肠道中的积累速度较慢。值得注意的是，暴露于PS NPs并没有引起显著的死亡率、畸形或线粒体生物能量学的变化，但确实降低了心率。最后，暴露于PS NPs会改变幼虫的行为，暴露的幼虫游泳活动减少就是证据。综上所述，这些数据表明，至少有一些纳米塑料可以穿透发育中的斑马鱼的绒毛膜，在组织中积累，并影响生理和行为，潜在地影响受污染水生生态系统中的生物适应性。
Plastic pollution is a critical environmental concern and comprises the majority of anthropogenic debris in the ocean, including macro, micro, and likely nano-scale (less than 100 nm in at least one dimension) plastic particles. While the toxicity of macroplastics and microplastics is relatively well studied, the toxicity of nanoplastics is largely uncharacterized. Here, fluorescent polystyrene nanoparticles (PS NPs) were used to investigate the potential toxicity of nanoplastics in developing zebrafish (Danio rerio), as well as characterize the uptake and distribution of the particles within embryos and larvae. Zebrafish embryos at 6 h post-fertilization (hpf) were exposed to PS NPs (0.1, 1, or 10 ppm) until 120 hpf. Our results demonstrate that PS NPs accumulated in the yolk sac as early as 24 hpf and migrated to the gastrointestinal tract, gallbladder, liver, pancreas, heart, and brain throughout development (48 hpf-120 hpf). Accumulation of PS NPs decreased during the depuration phase (120 hpf-168 hpf) in all organs, but at a slower rate in the pancreas and gastrointestinal tract. Notably, exposure to PS NPs did not induce significant mortality, deformities, or changes to mitochondrial bioenergetics, but did decrease the heart rate. Lastly, exposure to PS NPs altered larval behavior as evidenced by swimming hypoactivity in exposed larvae. Taken together, these data suggest that at least some nanoplastics can penetrate the chorion of developing zebrafish, accumulate in the tissues, and affect physiology and behavior, potentially affecting organismal fitness in contaminated aquatic ecosystems.
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影响因子：
2.9
作者：
Brown DR;Bailey JM;Oliveri AN;Levin ED;Di Giulio RT
通讯作者：
Di Giulio RT
影响因子：
3.7
作者：
Stackley KD;Beeson CC;Rahn JJ;Chan SS
通讯作者：
Chan SS
影响因子：
4.5
作者：
Manabe, Masahiko;Tatarazako, Norihisa;Kinoshita, Masato
通讯作者：
Kinoshita, Masato
影响因子：
11.4
作者：
Mattsson, Karin;Ekvall, Mikael T.;Cedervall, Tommy
通讯作者：
Cedervall, Tommy
DOI：
10.1098/rstb.2008.0205
发表时间：
2009-07-27
影响因子：
6.3
作者：
Barnes, David K. A.;Galgani, Francois;Barlaz, Morton
通讯作者：
Barlaz, Morton