Effects of Growth Hormone Receptor Ablation in Corticotropin-Releasing Hormone Cells.
Effects of Growth Hormone Receptor Ablation in Corticotropin-Releasing Hormone Cells.
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促肾上腺皮质激素释放激素细胞中生长激素受体消融的影响
DOI：
10.3390/ijms22189908
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发表时间：
2021-09-14
影响因子：
5.6
通讯作者：
Donato J Jr
中科院分区：
文献类型：
--
作者：
Dos Santos WO;Gusmao DO;Wasinski F;List EO;Kopchick JJ;Donato J Jr
促肾上腺皮质激素释放激素（CRH）细胞是下丘脑室旁核（PVH）中对生长激素（GH）反应的主要神经元。然而，生长激素受体（GHR）信号在CRH神经元的生理重要性是目前未知的。因此，本研究的主要目的是研究雄性和雌性小鼠CRH表达细胞中GHR消融的后果。CRH细胞中GHR消融未引起体重、身体组成、食物摄入、底物氧化、自发活动、葡萄糖耐量、胰岛素敏感性、对2-脱氧-D-葡萄糖的反调节反应和ghrelin诱导的食物摄入的显著变化。然而，在CRH细胞中进行GHR消融的雌性小鼠中观察到能量消耗减少。CRH细胞中GHR的缺失不影响雄性和雌性小鼠的焦虑、昼夜糖皮质激素水平或限制应激诱导的皮质酮分泌和PVH神经元的激活。总之，GHR消融，特别是在CRH表达神经元中，不会导致小鼠代谢、下丘脑-垂体-肾上腺轴、急性应激反应或焦虑的重大改变。考虑到以往的研究表明，中央GHR信号调节稳态的情况下，代谢应激，未来的研究仍然是必要的，以确定潜在的生理重要性，生长激素对CRH神经元的行动。
Corticotropin-releasing hormone (CRH) cells are the dominant neuronal population responsive to the growth hormone (GH) in the paraventricular nucleus of the hypothalamus (PVH). However, the physiological importance of GH receptor (GHR) signaling in CRH neurons is currently unknown. Thus, the main objective of the present study was to investigate the consequences of GHR ablation in CRH-expressing cells of male and female mice. GHR ablation in CRH cells did not cause significant changes in body weight, body composition, food intake, substrate oxidation, locomotor activity, glucose tolerance, insulin sensitivity, counterregulatory response to 2-deoxy-D-glucose and ghrelin-induced food intake. However, reduced energy expenditure was observed in female mice carrying GHR ablation in CRH cells. The absence of GHR in CRH cells did not affect anxiety, circadian glucocorticoid levels or restraint-stress-induced corticosterone secretion and activation of PVH neurons in both male and female mice. In summary, GHR ablation, specifically in CRH-expressing neurons, does not lead to major alterations in metabolism, hypothalamic–pituitary–adrenal axis, acute stress response or anxiety in mice. Considering the previous studies showing that central GHR signaling regulates homeostasis in situations of metabolic stress, future studies are still necessary to identify the potential physiological importance of GH action on CRH neurons.
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CRH 刺激健康、非肥胖成年人的皮质醇释放和食物摄入。
影响因子：
3.7
作者：
George, Sophie A.;Khan, Samir;Briggs, Hedieh;Abelson, James L.
通讯作者：
Abelson, James L.
影响因子：
5.3
作者：
Dos-Santos RC;Grover HM;Reis LC;Ferguson AV;Mecawi AS
通讯作者：
Mecawi AS
DOI：
10.1016/j.pnpbp.2019.109730
发表时间：
2020-01-10
影响因子：
5.6
作者：
Donner, Nina C.;Davies, Sofia M.;Lowry, Christopher A.
通讯作者：
Lowry, Christopher A.
影响因子：
3.5
作者：
Furigo, Isadora C.;Teixeira, Pryscila D. S.;Donato Jr, Jose
通讯作者：
Donato Jr, Jose
影响因子：
2.9
作者：
Gatford, Kathryn L.;Muhlhausler, Beverly S.;Chen, Chen
通讯作者：
Chen, Chen