Deficiency of the eIF4E isoform nCBP limits the cell-to-cell movement of a plant virus encoding triple-gene-block proteins in Arabidopsis thaliana.
Deficiency of the eIF4E isoform nCBP limits the cell-to-cell movement of a plant virus encoding triple-gene-block proteins in Arabidopsis thaliana.
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DOI：
10.1038/srep39678
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发表时间：
2017-01-06
影响因子：
4.6
通讯作者：
Namba S
中科院分区：
文献类型：
--
作者：
Keima T;Hagiwara-Komoda Y;Hashimoto M;Neriya Y;Koinuma H;Iwabuchi N;Nishida S;Yamaji Y;Namba S
隐性抗性是作物抗病毒育种的重要遗传策略之一。两个真核翻译起始因子4 E家族基因eIF 4 E和eIFiso 4 E是最常见的隐性抗性基因，其缺失抑制马铃薯Y病毒科、卡莫病毒和黄瓜病毒属中的植物病毒的感染。在这里，我们表明，另一个eIF 4 E家族基因，nCBP，作为一种新的隐性抗性基因在拟南芥对植物病毒的α-和β-flexiviridae。我们发现亚洲车前花叶病毒（Plantagoasiaticamosaicvirus，PlAMV）的侵染在亚洲车前ncbp突变体中延迟。thaliana.在单细胞中，ncbp突变体和野生型植物之间的病毒复制效率没有差异，但在ncbp突变体中，病毒的细胞间运动显著延迟。此外，三基因块蛋白2（TGB 2）和TGB 3，马铃薯X病毒的运动蛋白的积累，减少在ncbp突变体。用几种病毒进行的接种实验表明，在ncbp突变体中，编码TGB的病毒在其基因组中的积累减少。这些结果表明，nCBP是eIF 4 E家族隐性抗性基因的新成员，其缺失通过抑制TGB 2和TGB 3的有效积累而损害病毒细胞间的移动。
One of the important antiviral genetic strategies used in crop breeding is recessive resistance. Two eukaryotic translation initiation factor 4E family genes, eIF4E and eIFiso4E, are the most common recessive resistance genes whose absence inhibits infection by plant viruses in Potyviridae, Carmovirus, and Cucumovirus. Here, we show that another eIF4E family gene, nCBP, acts as a novel recessive resistance gene in Arabidopsis thaliana toward plant viruses in Alpha- and Betaflexiviridae. We found that infection by Plantago asiatica mosaic virus (PlAMV), a potexvirus, was delayed in ncbp mutants of A. thaliana. Virus replication efficiency did not differ between an ncbp mutant and a wild type plant in single cells, but viral cell-to-cell movement was significantly delayed in the ncbp mutant. Furthermore, the accumulation of triple-gene-block protein 2 (TGB2) and TGB3, the movement proteins of potexviruses, decreased in the ncbp mutant. Inoculation experiments with several viruses showed that the accumulation of viruses encoding TGBs in their genomes decreased in the ncbp mutant. These results indicate that nCBP is a novel member of the eIF4E family recessive resistance genes whose loss impairs viral cell-to-cell movement by inhibiting the efficient accumulation of TGB2 and TGB3.
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影响因子：
6.7
作者：
Chou YL;Hung YJ;Tseng YH;Hsu HT;Yang JY;Wung CH;Lin NS;Meng M;Hsu YH;Chang BY
通讯作者：
Chang BY
影响因子：
3.5
作者：
DOLJA, VV;GRAMA, DP;ATABEKOV, JG
通讯作者：
ATABEKOV, JG
影响因子：
3.4
作者：
Joshi, B;Lee, K;Maeder, DL;Jagus, R
通讯作者：
Jagus, R
影响因子：
3.7
作者：
Himeno, Misako;Maejima, Kensaku;Namba, Shigetou
通讯作者：
Namba, Shigetou
影响因子：
11.6
作者：
Jin, JB;Kim, YA;Hwang, I
通讯作者：
Hwang, I