Dynamic alteration of the colonic microbiota in intestinal ischemia-reperfusion injury.
Dynamic alteration of the colonic microbiota in intestinal ischemia-reperfusion injury.
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DOI：
10.1371/journal.pone.0042027
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Li J
中科院分区：
文献类型：
--
作者：
Wang F;Li Q;Wang C;Tang C;Li J
肠道缺血再灌注（I/R）在危重疾病中起着重要作用。肠道菌群参与损伤的发病机制。本研究旨在揭示结肠微生物群的改变模式，识别显着差异的特定细菌种类，并观察再灌注过程中同一损伤模型中结肠上皮的变化。采用变性梯度凝胶电泳（DGGE）监测分别经历0.5小时缺血和再灌注后1、3、6、12、24和72小时的对照大鼠和实验大鼠的结肠微生物群。基于 DGGE 图谱，结合统计方法估计了微生物群的相似性、细菌多样性和表征菌群失调的物种。对凝胶中感兴趣的细菌种类进行切割和测序，随后通过实时 PCR 进行定量和确认。同时，通过显微镜和D-乳酸分析检查上皮屏障。结肠菌群早期发生变化，并在再灌注后 6 小时出现显着差异，然后开始恢复。这些变化的特点是大肠杆菌和口腔普雷沃氏菌的增加，以及乳酸杆菌的增殖和上皮的愈合。这项研究首次表明，肠道缺血再灌注会导致上皮损伤后的结肠菌群失调，并确定了影响最大的细菌种类。
Intestinal ischemia-reperfusion (I/R) plays an important role in critical illnesses. Gut flora participate in the pathogenesis of the injury. This study is aimed at unraveling colonic microbiota alteration pattern and identifying specific bacterial species that differ significantly as well as observing colonic epithelium change in the same injury model during the reperfusion time course. Denaturing gradient gel electrophoresis (DGGE) was used to monitor the colonic microbiota of control rats and experimental rats that underwent 0.5 hour ischemia and 1, 3, 6, 12, 24, and 72 hours following reperfusion respectively. The microbiota similarity, bacterial diversity and species that characterized the dysbiosis were estimated based on the DGGE profiles using a combination of statistical approaches. The interested bacterial species in the gel were cut and sequenced and were subsequently quantified and confirmed with real-time PCR. Meanwhile, the epithelial barrier was checked by microscopy and D-lactate analysis. Colonic flora changed early and differed significantly at 6 hours after reperfusion and then started to recover. The shifts were characterized by the increase of Escherichia coli and Prevotella oralis, and Lactobacilli proliferation together with epithelia healing. This study shows for the first time that intestinal ischemia-reperfusion results in colonic flora dysbiosis that follows epithelia damage, and identifies the bacterial species that contribute most.
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影响因子：
5
作者：
Di Paola, R;Mazzon, E;Cuzzocrea, S
通讯作者：
Cuzzocrea, S
影响因子：
2.4
作者：
Garcez, Aguinaldo S.;Ribeiro, Martha S.;Hamblin, Michael R.
通讯作者：
Hamblin, Michael R.
影响因子：
3.8
作者：
Diebel, LN;Liberati, DM;Brown, WJ
通讯作者：
Brown, WJ
DOI：
10.1097/00005373-200201000-00005
发表时间：
2002-01-01
影响因子：
--
作者：
Doty, JM;Oda, J;Sugerman, HJ
通讯作者：
Sugerman, HJ
影响因子：
56.9
作者：
Gill, Steven R.;Pop, Mihai;Nelson, Karen E.
通讯作者：
Nelson, Karen E.