Spatial constraints dictate glial territories at murine neuromuscular junctions.
Spatial constraints dictate glial territories at murine neuromuscular junctions.
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DOI：
10.1083/jcb.201108005
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发表时间：
2011-10-17
期刊：
影响因子：
--
通讯作者：
Misgeld T
中科院分区：
文献类型：
--
作者：
Brill MS;Lichtman JW;Thompson W;Zuo Y;Misgeld T
神经胶质细胞之间的空间竞争导致它们将神经肌肉连接划分为突触内的离散区域。雪旺细胞(SCs)是周围神经系统的神经胶质细胞，覆盖突触终末，使它们能够监测和调节神经传递。神经胶质细胞覆盖的中断会导致轴突变性和突触丢失。建立和维持这一覆盖率的细胞机制在很大程度上仍不清楚。为了解决这个问题，我们标记了单个干细胞并进行了时间推移成像实验。成年的末端SC以静态的瓦片图案排列，而年轻的SC动态地混合。发育中胶质细胞分离的机制似乎是空间竞争，其中神经胶质细胞和轴突神经胶质细胞的接触限制了单个SCs的范围，四种类型的实验表明：(1)激光消融单个SCs，导致相邻SCs的领土立即扩大；(2)横断轴突，导致成年SCs动态地混合；(3)轴突断裂受阻的突变小鼠的轴突切断，其中混合被推迟；以及(4)活动阻断，没有立即的影响。综上所述，我们得出结论，神经胶质细胞通过竞争突触周围空间来划分突触。
Spatial competition between glial cells causes them to partition neuromuscular junctions into discrete domains within a synapse. Schwann cells (SCs), the glial cells of the peripheral nervous system, cover synaptic terminals, allowing them to monitor and modulate neurotransmission. Disruption of glial coverage leads to axon degeneration and synapse loss. The cellular mechanisms that establish and maintain this coverage remain largely unknown. To address this, we labeled single SCs and performed time-lapse imaging experiments. Adult terminal SCs are arranged in static tile patterns, whereas young SCs dynamically intermingle. The mechanism of developmental glial segregation appears to be spatial competition, in which glial–glial and axonal–glial contacts constrain the territory of single SCs, as shown by four types of experiments: (1) laser ablation of single SCs, which led to immediate territory expansion of neighboring SCs; (2) axon removal by transection, resulting in adult SCs intermingling dynamically; (3) axotomy in mutant mice with blocked axon fragmentation in which intermingling was delayed; and (4) activity blockade, which had no immediate effects. In summary, we conclude that glial cells partition synapses by competing for perisynaptic space.
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影响因子：
5.3
作者：
Haber, Michael;Zhou, Lei;Murai, Keith K.
通讯作者：
Murai, Keith K.
影响因子：
14.8
作者：
Kerschensteiner, Martin;Reuter, Miriam S.;Misgeld, Thomas
通讯作者：
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通过神经胶质调节突触连通性。
影响因子：
64.8
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作者：
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通讯作者：
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影响因子：
5.3
作者：
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通讯作者：
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