Sfh1, an essential component of the RSC chromatin remodeling complex, maintains genome integrity in fission yeast
Sfh1, an essential component of the RSC chromatin remodeling complex, maintains genome integrity in fission yeast
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Sfh1 是 RSC 染色质重塑复合物的重要组成部分，可维持裂殖酵母基因组的完整性
DOI：
10.1111/gtc.12629
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发表时间：
2018
期刊：
影响因子：
2.1
通讯作者：
Naoe Kotomura Satoru Tsunemine Masahiro Kuragano Takahiro Asanuma Hiromi Nakagawa Katsunori Tanaka Yota Murakami
中科院分区：
文献类型：
--
作者：
Naoe Kotomura Satoru Tsunemine Masahiro Kuragano Takahiro Asanuma Hiromi Nakagawa Katsunori Tanaka Yota Murakami
Abp1是一种裂变酵母的CENP - B同源物，它有助于着丝粒功能，在着丝粒周围异染色质和反转录转座子的沉默。我们确定了fh11基因，该基因编码分裂酵母染色质重塑复合体RSC的一个核心亚基，作为Abp1‐相互作用蛋白。由于fh11对生长至关重要，我们分离出温度敏感的h1突变体。这些突变体表现出着丝粒功能的缺陷，反映在对纺锤体形成抑制剂和小染色体不稳定性的敏感性上。Sfh1定位于着丝点和周围着丝点异染色质区域。尽管sfh11突变对这些区域的沉默影响较小，但它们降低了着丝粒异染色质上粘接蛋白的水平。Sfh1也以部分依赖Abp1的方式定位在反转录转座子Tf2上，并可能在与组蛋白伴侣HIRA相同的途径上帮助Abp1沉默Tf2， HIRA也参与抑制intf2。此外，sfh1突变体对几种DNA损伤处理（HU、MMS、UV和X射线）敏感。重组中介蛋白Rad52同系物Rad22的自发位点增加，表明RSC在Rad22募集下游的双链断裂的同源重组修复中起作用。这些结果表明，RSC在维持基因组完整性方面发挥着多种作用。
Abp1 is a fission yeast CENP‐B homologue that contributes to centromere function, silencing at pericentromeric heterochromatin and silencing of retrotransposons. We identified thesfh1gene, encoding a core subunit of the fission yeast chromatin remodeling complex RSC as an Abp1‐interacting protein. Becausesfh1is essential for growth, we isolated temperature‐sensitivesfh1mutants. These mutants showed defects in centromere functions, reflected by sensitivity to an inhibitor of spindle formation and minichromosome instability. Sfh1 localized at both kinetochore and pericentromeric heterochromatin regions. Althoughsfh1mutations had minor effect on silencing at these regions, they decreased the levels of cohesin on centromeric heterochromatin. Sfh1 also localized at a retrotransposon,Tf2, in a partly Abp1‐dependent manner, and assisted in silencing ofTf2by Abp1 probably in the same pathway as a histone chaperon, HIRA, which is also known to involve inTf2repression. Furthermore,sfh1mutants were sensitive to several DNA‐damaging treatments (HU, MMS, UV and X‐ray). Increase in spontaneous foci of Rad22, a recombination Mediator protein Rad52 homologue, insfh1mutant suggests that RSC functions in homologous recombination repair of double‐stranded break downstream of the Rad22 recruitment. These results indicate that RSC plays multiple roles in the maintenance of genome integrity.
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影响因子：
16
作者：
Badis, Gwenael;Chan, Esther T.;van Bakel, Harm;Pena-Castillo, Lourdes;Tillo, Desiree;Tsui, Kyle;Carlson, Clayton D.;Gossett, Andrea J.;Hasinoff, Michael J.;Warren, Christopher L.;Gebbia, Marinella;Talukder, Shaheynoor;Yang, Ally;Mnaimneh, Sanie;Terterov, Dimitri;Coburn, David;Yeo, Ai Li;Yeo, Zhen Xuan;Clarke, Neil D.;Lieb, Jason D.;Ansari, Aseem Z.;Nislow, Corey;Hughes, Timothy R.
通讯作者：
Hughes, Timothy R.
影响因子：
16
作者：
Huang, J;Hsu, JM;Laurent, BC
通讯作者：
Laurent, BC
影响因子：
3.3
作者：
Takayama, Yuko;Sato, Hiroshi;Takahashi, Kohta
通讯作者：
Takahashi, Kohta
影响因子：
2.1
作者：
Dohke, Kohei;Miyazaki, Shota;Murakami, Yota
通讯作者：
Murakami, Yota
通过使用替代转录起始位点调节逆转录转座子活性
影响因子：
7.7
作者：
Jenna Persson;Babett Steglich;Agata Smialowska;Mette Boyd;Jette Bornholdt;R. Andersson;C. Schurra;B. Arcangioli;A. Sandelin;O. Nielsen;K. Ekwall
通讯作者：
K. Ekwall