Detection of exosomal biomarker by electric field-induced release and measurement (EFIRM).
Detection of exosomal biomarker by electric field-induced release and measurement (EFIRM).
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DOI：
10.1016/j.bios.2012.12.046
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发表时间：
2013-06-15
影响因子：
12.6
通讯作者：
Wong, David T. W.
中科院分区：
文献类型：
--
作者：
Wei, Fang;Yang, Jieping;Wong, David T. W.
外泌体生物标志物介导重要的生物学过程，特别是在全身性疾病的诊断和治疗中，但保护性的外泌体囊泡结构阻碍了对所携带分子的快速、简单检测。我们建立了一种新的方法——电场诱导释放和测量（EFIRM），该方法可以同时破坏外泌体释放内容物并现场监测所携带的外泌体RNA/蛋白质生物标志物。当暴露于非均匀电场时，外泌体RNA和蛋白质迅速释放。这些生物分子的生物识别是同时进行的。我们验证了一个假设，即稳定转染hCD63-GFP的肺癌细胞系H460会脱落表达hCD63-GFP的外泌体，这些外泌体可以在血清和唾液中检测到。我们在体内证实H460-CD63-GFP脱落外泌体被转运到血液和唾液中。这一结果表明，除了血液外，还首次在唾液中检测到肿瘤脱落的外泌体，这为基于外泌体的唾液生物标志物检测提供了一种新的翻译应用。
Exosomes biomarkers mediating important biological process, especially in the systemic disease diagnostics and therapeutics, yet the protective exosomal vesicle structure hinders rapid, simple detection of the harbored molecules. We have established a new method, the electric field-induced release and measurement (EFIRM), which can simultaneously disrupt exosomes to release the contents and on-site monitoring the harbored exosomal RNA/proteins biomarkers. When exposed to a nonuniform electrical field, exosomal RNA and proteins are rapidly released. Bio-recognition of these biomolecules is carried out concurrently. We tested the hypothesis that the lung cancer cell line, H460 stably transfected with hCD63-GFP, would shed hCD63-GFP expressing exosomes that could be detected in serum and saliva. We confirmed in vivo that H460-CD63-GFP shed exosomes were transported to blood and saliva. This result demonstrates for the first time tumor-shed exosomes were detected in saliva, in addition to blood, presenting a new translational utility of exosome-based biomarker detection in saliva.
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影响因子：
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