Microfluidic isolation and transcriptome analysis of serum microvesicles.
Microfluidic isolation and transcriptome analysis of serum microvesicles.
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DOI：
10.1039/b916199f
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发表时间：
2010-02-21
期刊：
影响因子：
6.1
通讯作者：
Irimia D
中科院分区：
文献类型：
--
作者：
Chen C;Skog J;Hsu CH;Lessard RT;Balaj L;Wurdinger T;Carter BS;Breakefield XO;Toner M;Irimia D
从正常细胞和癌细胞脱落的微泡（外来体）可以作为细胞间通讯的手段。这些微泡携带来自宿主细胞的蛋白质、脂质和核酸。它们从血液样品中的分离和分析有可能提供关于恶性肿瘤的状态和进展的信息，并且应该证明作为各种疾病状态的生物标志物具有重要的临床意义。然而，目前用于从血液中分离微泡的方案需要高速离心和过滤，这是繁琐且耗时的。为了充分利用微泡作为临床应用生物标志物的潜力，将需要更快、更简单的分离方法。在本文中，我们提出了一种简单，快速的微流控免疫亲和方法，从小体积的血清从血液样品和条件培养基从培养细胞中分离微泡。可以从这些微泡中提取高质量的RNA，提供有关肿瘤遗传状态的信息来源，以用作癌症诊断和预后的生物标志物。
Microvesicles (exosomes) shed from both normal and cancerous cells may serve as means of intercellular communication. These microvesicles carry proteins, lipids and nucleic acids derived from the host cell. Their isolation and analysis from blood samples have the potential to provide information about state and progression of malignancy and should prove of great clinical importance as biomarkers for a variety of disease states. However, current protocols for isolation of microvesicles from blood require high-speed centrifugation and filtration, which are cumbersome and time consuming. In order to take full advantage of the potential of microvesicles as biomarkers for clinical applications, faster and simpler methods of isolation will be needed. In this paper, we present an easy and rapid microfluidic immunoaffinity method to isolate microvesicles from small volumes of both serum from blood samples and conditioned medium from cells in culture. RNA of high quality can be extracted from these microvesicles providing a source of information about the genetic status of tumors to serve as biomarkers for diagnosis and prognosis of cancer.
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影响因子：
9.3
作者：
Russom, Aman;Sethu, Palaniappan;Toner, Mehmet
通讯作者：
Toner, Mehmet
影响因子：
6.1
作者：
Choi, Sungyoung;Park, Je-Kyun
通讯作者：
Park, Je-Kyun
影响因子：
2.2
作者：
Lamparski, HG;Metha-Damani, A;Le Pecq, JB
通讯作者：
Le Pecq, JB
DOI：
10.1126/science.1164382
发表时间：
2008-09-26
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Parsons DW;Jones S;Zhang X;Lin JC;Leary RJ;Angenendt P;Mankoo P;Carter H;Siu IM;Gallia GL;Olivi A;McLendon R;Rasheed BA;Keir S;Nikolskaya T;Nikolsky Y;Busam DA;Tekleab H;Diaz LA Jr;Hartigan J;Smith DR;Strausberg RL;Marie SK;Shinjo SM;Yan H;Riggins GJ;Bigner DD;Karchin R;Papadopoulos N;Parmigiani G;Vogelstein B;Velculescu VE;Kinzler KW
通讯作者：
Kinzler KW
DOI：
10.1083/jcb.200508014
发表时间：
2006-03-13
期刊：
The Journal of cell biology
影响因子：
--
作者：
Booth AM;Fang Y;Fallon JK;Yang JM;Hildreth JE;Gould SJ
通讯作者：
Gould SJ