3-D Human Renal Tubular Organoids Generated from Urine-Derived Stem Cells for Nephrotoxicity Screening.
3-D Human Renal Tubular Organoids Generated from Urine-Derived Stem Cells for Nephrotoxicity Screening.
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DOI：
10.1021/acsbiomaterials.0c01468
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发表时间：
2020-12-14
影响因子：
5.8
通讯作者：
Zhang Y
中科院分区：
文献类型：
--
作者：
Guo H;Deng N;Dou L;Ding H;Criswell T;Atala A;Furdui CM;Zhang Y
迫切需要开发基于人类细胞的系统来取代啮齿类动物或细胞的二维培养来研究肾毒性。人尿源干细胞经肾细胞外基质诱导，在三维培养中分化为肾小管上皮细胞。3-D类器官中CYP2E1和KIM-1水平在丙酮和顺铂治疗后显著升高。这种三维培养系统为肾毒性筛选和研究提供了另一种工具。
The development of human cell-based systems to replace the use of rodents or the two-dimensional culture of cells for studying nephrotoxicity is urgently needed. Human urine-derived stem cells were differentiated into renal tubular epithelial cells in three-dimensional (3-D) culture after being induced by a kidney extracellular matrix. Levels of CYP2E1 and KIM-1 in 3-D organoids were significantly increased in response to acetone and cisplatin. This 3-D culture system provides an alternative tool for nephrotoxicity screening and research.
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影响因子：
4.1
作者：
Bharadwaj, Shantaram;Liu, Guihua;Zhang, Yuanyuan
通讯作者：
Zhang, Yuanyuan
影响因子：
14
作者：
Astashkina, Anna I.;Mann, Brenda K.;Grainger, David W.
通讯作者：
Grainger, David W.
影响因子：
10
作者：
Ali, Mohamed;Kumar, Anil P. R.;Lee, Sang Jin
通讯作者：
Lee, Sang Jin
影响因子：
4.9
作者：
Li, Yao;Kandasamy, Karthikeyan;Zink, Daniele
通讯作者：
Zink, Daniele
DOI：
10.1111/j.1440-1746.2006.04595.x
发表时间：
2006-10-01
影响因子：
4.1
作者：
Cederbaum, Arthur I.
通讯作者：
Cederbaum, Arthur I.