Comparison of Intra-CL Injection and Peripheral Application of Prostaglandin F(2α) Analog on Luteal Blood Flow and Secretory Function of the Bovine Corpus Luteum.
Comparison of Intra-CL Injection and Peripheral Application of Prostaglandin F(2α) Analog on Luteal Blood Flow and Secretory Function of the Bovine Corpus Luteum.
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DOI：
10.3389/fvets.2021.811809
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发表时间：
2021
影响因子：
3.2
通讯作者：
Skarzynski DJ
中科院分区：
文献类型：
--
作者：
Jonczyk AW;Piotrowska-Tomala KK;Skarzynski DJ
我们研究了不同剂量的地诺前列素直接注射到牛黄体（CL）中对（i）外周血中孕酮（P4）和催产素（OT）浓度以及（ii）类固醇生成急性调节蛋白（星星）、细胞色素P450家族11亚家族A成员1（P450 SCC）、羟基-δ-5-类固醇脱氢酶、CL组织中的3 β和类固醇δ异构酶1（HSD 3B）以及受体相互作用蛋白激酶1和3（RIPK 1、RIPK 3）。此外，我们还检查了地诺前列素CL内注射或肌内（IM）给药对CL分泌功能和CL血管网络状态指标的影响：黄体组织面积（LTA）、CL血流量（CLBF）和黄体早期和中期奶牛的CLBF：LTA比值（CLBF. CLBF）。在实验1中，奶牛（周期的第10天）被分配到（i）CL内注射盐水（对照; n = 3）;（ii）CL内注射地诺前列素（1.25 mg; 2.5 mg或5 mg;每个剂量n = 3）;（iii）IM给予盐水（对照; n = 3）;或（iv）地诺前列素的IM施用（25 mg;阳性对照; n = 3）。测量血浆样品中OT和P4的浓度。类固醇生成或坏死性凋亡相关因子的mRNA表达测定CL组织4小时后的治疗。在实验2中，将第4天（n = 12）或第10天（n = 12）的奶牛分配至（i）CL内注射地诺前列素（2.5 mg/0.5 ml; n = 6）或（ii）IM给予地诺前列素（25 mg/5 ml; n = 6）。测量血浆样品中的P4浓度。根据彩色多普勒超声检查评估黄体组织面积、CLBF和CLBF。在黄体中期，CL内注射地诺前列素以剂量依赖性方式增加了奶牛颈静脉（JV）中的OT水平并降低了P4水平。在中期CL中，CL内注射地诺前列素后2小时观察到CLBF和CLBF增加，伴有P4水平降低。星星降低和RIPK 1和RIPK 3 mRNA水平升高证实，2.5 mg地诺前列素直接注射至CL是诱导黄体溶解级联反应的最小剂量。地诺前列素直接注射至CL（剂量低于外周应用的推荐剂量）导致与IM地诺前列素给药相似的模式。
We investigated the effects of different doses of dinoprost injected directly into the bovine corpus luteum (CL) on (i) concentrations of progesterone (P4) and oxytocin (OT) in peripheral blood and (ii) mRNA levels of steroidogenic acute regulatory protein (STAR), cytochrome P450 family 11 subfamily A member 1 (P450scc), hydroxy-delta-5-steroid dehydrogenase, 3 β- and steroid delta-isomerase 1 (HSD3B), and receptor-interacting protein kinases 1 and 3 (RIPK1, RIPK3) in CL tissue. Moreover, we examined the effects of dinoprost, injected intra-CL or administered intramuscularly (IM), on CL secretory function and on indicators of CL vascular network status: luteal tissue area (LTA), CL blood flow (CLBF), and the CLBF:LTA ratio (Adj. CLBF), in cows at the early and mid-luteal phases. In the Experiment 1, cows (day 10 of the cycle) were allocated to (i) an intra-CL injection of saline (control; n = 3); (ii) an intra-CL injection of dinoprost (1.25 mg; 2.5 mg, or 5 mg; n = 3 for each dose); (iii) an IM administration of saline (control; n = 3); or (iv) an IM administration of dinoprost (25 mg; positive control; n = 3). Concentrations of OT and P4 were measured in plasma samples. The mRNA expression of steroidogenesis- or necroptosis-related factors was determined in CL tissue 4 h after treatments. In Experiment 2, cows on day 4 (n = 12) or day 10 (n = 12) were allocated to (i) an intra-CL injection of dinoprost (2.5 mg/0.5 ml; n = 6), or (ii) IM administration of dinoprost (25 mg/5 ml; n = 6). Concentrations of P4 were measured in plasma samples. Luteal tissue area, CLBF, and Adj. CLBF were assessed based on color Doppler ultrasonography. An intra-CL injection of dinoprost increased OT and decreased P4 levels in the jugular vein (JV) in a dose-dependent manner in cows at the mid-luteal phase. Increased CLBF and Adj. CLBF, accompanied by reduced P4 levels, were observed 2 h after intra-CL dinoprost injection in middle-stage CL. Decreased STAR and increased RIPK1 and RIPK3 mRNA levels confirmed that 2.5 mg dinoprost injected directly into CL is the minimum dose that induces luteolytic cascade. Injection of dinoprost directly into the CL (at a dosage lower than recommended for peripheral application) results in a pattern similar to IM dinoprost administration.
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影响因子：
1.8
作者：
Acosta, Tomas Javier;Bah, Mamadou Bousa;Skarzynski, Dariusz Jan
通讯作者：
Skarzynski, Dariusz Jan
影响因子：
1.8
作者：
Korzekwa, Anna J.;Okuda, Kiyoshi;Skarzynski, Dariusz J.
通讯作者：
Skarzynski, Dariusz J.
影响因子：
4.6
作者：
Hojo T;Siemieniuch MJ;Lukasik K;Piotrowska-Tomala KK;Jonczyk AW;Okuda K;Skarzynski DJ
通讯作者：
Skarzynski DJ
影响因子：
3.6
作者：
Atli, Mehmet O.;Bender, Robb W.;Wiltbank, Milo C.
通讯作者：
Wiltbank, Milo C.
影响因子：
2.8
作者：
Hojo, Takuo;Piotrowska-Tomala, Katarzyna K.;Skarzynski, Dariusz J.
通讯作者：
Skarzynski, Dariusz J.