Selection of a human T helper type 1-like T cell subset by mycobacteria.
Selection of a human T helper type 1-like T cell subset by mycobacteria.
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DOI：
10.1084/jem.174.3.583
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发表时间：
1991-09-01
期刊：
影响因子：
--
通讯作者：
Spits H
中科院分区：
文献类型：
--
作者：
Haanen JB;de Waal Malefijt R;Res PC;Kraakman EM;Ottenhoff TH;de Vries RR;Spits H
分枝杆菌在其宿主中引起细胞免疫应答。这种反应通常导致保护性免疫，但有时可能伴随着由于迟发型超敏反应（DTH）引起的免疫病理学。人类的一个突出例子是结核样麻风，其特征是对麻风分枝杆菌的高细胞免疫和由于DTH引起的免疫病理学。该病患者的皮肤病变具有DTH反应的特征，其中巨噬细胞和CD4 + T淋巴细胞占主导地位。在动物模型中，已经显示DTH应答与特定的CD4 + T细胞亚群（1型辅助性T细胞[Th1]）的存在相关，所述T细胞亚群仅分泌某些细胞因子，例如白细胞介素2（IL-2）、干扰素γ（IFN-γ）和光敏素，但不分泌IL-4或IL-5。我们研究了活化的M.来自结核样麻风患者的麻风反应性CD4 + T细胞克隆。这些与分枝杆菌热休克蛋白反应的T细胞克隆表现出Th1样细胞因子分泌模式，具有非常高水平的IFN-γ。这些克隆中有一半分泌低水平的IL-4和IL-5，但IFN-γ与IL-4和IL-5的比例远高于与非分枝杆菌抗原反应的T细胞克隆。一个Th1样细胞因子分泌模式也观察到T细胞克隆和多克隆T细胞系从控制个人承认热休克和其他分枝杆菌抗原。然而，这些克隆分泌的IFN-γ水平显着低于患者来源的T细胞克隆。这种Th1样的模式没有发现与T细胞克隆从相同的患者和健康人以相同的方式产生，但与非分枝杆菌抗原反应。因此，我们的数据表明，分枝杆菌选择性诱导人类T细胞与Th1样细胞因子分泌谱。
Mycobacteria elicit a cellular immune response in their hosts. This response usually leads to protective immunity, but may sometimes be accompanied by immunopathology due to delayed type hypersensitivity (DTH). A striking example in man is tuberculoid leprosy, which is characterized by high cellular immunity to Mycobacterium leprae and immunopathology due to DTH. Skin lesions of patients suffering from this disease have the characteristics of DTH reactions in which macrophages and CD4+ T lymphocytes predominate. In animal models, it has been shown that DTH responses are associated with the presence of a particular subset of CD4+ T cells (T helper type 1 [Th1]) that secrete only certain cytokines, such as interleukin 2 (IL-2), interferon gamma (IFN-gamma), and lymphotoxin, but no IL-4 or IL-5. We studied the cytokine release of activated M. leprae-reactive CD4+ T cell clones derived from tuberculoid leprosy patients. These T cell clones, which were reactive with mycobacterial heat shock proteins, exhibited a Th1- like cytokine secretion pattern with very high levels of IFN-gamma. Half of these clones secreted low levels of IL-4 and IL-5, but the ratio of IFN-gamma to IL-4 and IL-5 was much higher than that of T cell clones reactive with nonmycobacterial antigens. A Th1-like cytokine secretion pattern was also observed for T cell clones and polyclonal T cell lines from control individuals that recognized both heat shock and other mycobacterial antigens. The levels of IFN-gamma secreted by these clones were, however, significantly less than those of patient-derived T cell clones. This Th1-like pattern was not found with T cell clones from the same patients and healthy individuals generated in the same manner, but reactive with nonmycobacterial antigens. Our data thus indicate that mycobacteria selectively induce human T cells with a Th1- like cytokine secretion profile.
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影响因子：
3.7
作者：
HAANEN, JBAG;OTTENHOFF, THM;DEVRIES, RRP
通讯作者：
DEVRIES, RRP
影响因子：
15.3
作者：
OTTENHOFF, THM;NEUTEBOOM, S;DEVRIES, RRP
通讯作者：
DEVRIES, RRP
影响因子：
15.3
作者：
OTTENHOFF, THM;MUTIS, T
通讯作者：
MUTIS, T
DOI：
10.1016/s0769-2625(87)80030-2
发表时间：
1987-09-01
期刊：
ANNALES DE L INSTITUT PASTEUR-IMMUNOLOGY
影响因子：
--
作者：
LOCKSLEY, RM;HEINZEL, FP;GARDNER, KD
通讯作者：
GARDNER, KD
影响因子：
15.3
作者：
EHLERS, S;SMITH, KA
通讯作者：
SMITH, KA