Cytokine Profile and Immunoglobulin E-mediated Serological Food Hypersensitivity in Patients With Irritable Bowel Syndrome With Diarrhea.
Cytokine Profile and Immunoglobulin E-mediated Serological Food Hypersensitivity in Patients With Irritable Bowel Syndrome With Diarrhea.
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DOI：
10.5056/jnm17114
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发表时间：
2018-07-30
影响因子：
3.4
通讯作者：
Shiotani A
中科院分区：
文献类型：
--
作者：
Katsumata R;Ishii M;Lee S;Handa Y;Murao T;Fujita M;Matsumoto H;Otsuki T;Shiotani A
包括食物过敏在内的食物相互作用在肠易激综合征伴腹泻（IBS-D）的发病机制中起着关键作用。由于目前关于食物过敏与肠易激综合征之间关系的研究报道较少，本文旨在阐明肠易激综合征- d患者的血清学食物过敏的患病率，以及肠易激综合征- d患者的胃肠道症状和血清细胞因子特征以及血清学食物过敏。免疫球蛋白E （Ig E）介导的血清学食物超敏反应和血清细胞因子水平采用多重过敏原同步测试评估食物过敏原特异性血清IgE和Luminex Milliplex Panel包含多个荧光标记珠。2级及以上为ige介导的食物超敏反应阳性。采用胃肠症状评定量表对症状进行评定。我们招募了92名受试者，包括60名IBS-D患者和32名健康对照者。ige介导的血清学食物过敏患者的百分比在两组间无显著差异（对照组= 28.1%,IBS-D = 33.3%）。IBS-D患者血清IL-1β、IL-6、IL-8、巨噬细胞炎症蛋白-1 α和TNF-α水平均高于对照组。无ige介导的血清食物过敏患者血清TNF-α浓度（43.4 vs 21.8 pg/mL, P = 0.009）高于有食物过敏的IBS-D患者。三分之一的日本IBS-D患者表现为ige介导的血清学食物过敏。血清细胞因子谱不同，其特征是ige介导的血清学食物过敏的IBS-D患者炎症细胞因子水平较低。ige介导的食物超敏反应的血清学检测可以检测出某一群IBS-D。
Food interaction, including food hypersensitivity, plays a key role in the pathogenesis of irritable bowel syndrome with diarrhea (IBS-D). Since only a few studies have been reported about the relationship between food hypersensitivity and IBS-D, we elucidate the prevalence of serological food hypersensitivity in patients with IBS-D and the characteristics of gastrointestinal symptoms and serum cytokine profiles in patients with IBS-D and serological food hypersensitivity. Immunoglobulin E (Ig E)-mediated serological food hypersensitivity and serum cytokine levels were evaluated using the multiple allergen simultaneous test evaluating food allergen-specific serum IgE and Luminex Milliplex Panel containing multiple fluorescence-labeled beads. Class 2 or above was considered as IgE-mediated food hypersensitivity positive. The gastrointestinal symptom rating scale was used to evaluate symptoms. We enrolled 92 subjects, including 60 with IBS-D and 32 healthy controls. The percentages of patients with IgE-mediated serological food hypersensitivity were not significantly different between the groups (controls = 28.1% and IBS-D = 33.3%). Serum IL-1β, IL-6, IL-8, macrophage inflammatory protein-1alpha, and TNF-α levels were higher in patients with IBS-D than in controls. Serum concentration of TNF-α (43.4 vs 21.8 pg/mL, P = 0.009) was higher in patients with IBS-D without IgE-mediated serological food hypersensitivity than those with food hypersensitivity. One-third of Japanese patients with IBS-D showed IgE-mediated serological food hypersensitivity. The serum cytokine profile differed and was characterized by lower inflammatory cytokine levels in IBS-D with IgE-mediated serological food hypersensitivity. Serological test regarding IgE-mediated food hypersensitivity can detect a certain cluster of IBS-D.
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DOI：
10.1038/nrrheum.2015.169
发表时间：
2016-01
期刊：
Nature reviews. Rheumatology
影响因子：
--
作者：
Kalliolias GD;Ivashkiv LB
通讯作者：
Ivashkiv LB
DOI：
10.4049/jimmunol.1303026
发表时间：
2014-03-15
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Johnston LK;Chien KB;Bryce PJ
通讯作者：
Bryce PJ
影响因子：
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作者：
Han M;Shin S;Park H;Park KU;Park MH;Song EY
通讯作者：
Song EY
影响因子：
3.5
作者：
Bashashati, M.;Rezaei, N.;Simren, M.
通讯作者：
Simren, M.
影响因子：
7.6
作者：
Lillestol, K.;Helgeland, L.;Berstad, A.
通讯作者：
Berstad, A.