Repeat tick exposure elicits distinct immune responses in guinea pigs and mice.
Repeat tick exposure elicits distinct immune responses in guinea pigs and mice.
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DOI：
10.1016/j.ttbdis.2020.101529
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发表时间：
2020-11
影响因子：
3.2
通讯作者：
Fikrig E
中科院分区：
文献类型：
--
作者：
Kurokawa C;Narasimhan S;Vidyarthi A;Booth CJ;Mehta S;Meister L;Diktas H;Strank N;Lynn GE;DePonte K;Craft J;Fikrig E
蜱在叮咬过程中将存款唾液蛋白沉积到皮肤中以介导获得血餐。已证明对蜱叮咬的获得性抗性可预防广义伯氏疏螺旋体（s.l.）传输然而，耐药性的机制以及保护性抗原仍然难以捉摸。为了解决这些未知因素，我们利用了豚鼠模型的蜱阻力和小鼠模型的放纵。豚鼠在多次肩突硬蜱感染后产生了免疫力，其特征是蜱快速脱离和进食受损。相比之下，小鼠耐受至少6次感染，对喂养无显著影响。我们通过RNA测序和组织学分析了叮咬部位，确定了蜱免疫动物中的几种炎症途径，如FcεRI信号传导和补体激活，以及可能损害局部血流的凝血途径激活。总之，这些结果确定了在蜱虫排斥过程中改变的重要途径和可能作为候选疫苗的潜在蜱虫蛋白。
Ticks deposit salivary proteins into the skin during a bite to mediate acquisition of a blood meal. Acquired resistance to tick bites has been demonstrated to prevent Borrelia burgdorferi sensu lato (s.l.) transmission. However, the mechanism of resistance, as well as the protective antigens, have remained elusive. To address these unknowns, we utilized a guinea pig model of tick resistance and a mouse model of permissiveness. Guinea pigs developed immunity after multiple Ixodes scapularis tick infestations, characterized by rapid tick detachment and impaired feeding. In comparison, mice tolerated at least 6 infestations with no significant impact on feeding. We analyzed the bite sites by RNA-sequencing and histology, identifying several inflammatory pathways in tick immune animals, such as FcεRI signaling and complement activation, and activation of coagulation pathways that could impair local blood flow. Together, these results identify important pathways altered during tick rejection and potential tick proteins that could serve as vaccine candidates.
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影响因子：
6.7
作者：
Dai J;Narasimhan S;Zhang L;Liu L;Wang P;Fikrig E
通讯作者：
Fikrig E
影响因子：
3.2
作者：
Narasimhan, Sukanya;Kurokawa, Cheyne;Fikrig, Erol
通讯作者：
Fikrig, Erol
DOI：
10.1093/cid/ciu397
发表时间：
2014-09-01
期刊：
Clinical infectious diseases : an official publication of the Infectious Diseases Society of America
影响因子：
--
作者：
Hinckley AF;Connally NP;Meek JI;Johnson BJ;Kemperman MM;Feldman KA;White JL;Mead PS
通讯作者：
Mead PS
影响因子：
3.6
作者：
Glatz M;Means T;Haas J;Steere AC;Müllegger RR
通讯作者：
Müllegger RR
影响因子：
3.2
作者：
Maruyama, Sandra R.;Garcia, Gustavo R.;de Miranda-Santos, Isabel K. F.
通讯作者：
de Miranda-Santos, Isabel K. F.