Beyond editing to writing large genomes.
Beyond editing to writing large genomes.
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DOI：
10.1038/nrg.2017.59
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发表时间：
2017-12
期刊：
影响因子：
--
通讯作者：
Church GM
中科院分区：
文献类型：
--
作者：
Chari R;Church GM
最近基因组测序和工程技术的指数级进步使得人们对 DNA 变异（基因型）对细胞功能（表型）的影响的研究达到了前所未有的水平。此外，这些进步还引发了对书写和彻底重写复杂基因组的现实讨论。在本视角中，我们详细介绍了大规模工程的动机，讨论了细菌和酵母中此类项目所取得的进展，并描述了各种基因组工程技术将如何为这一努力做出贡献。最后，我们描述了理想平台的特征，并提供了促进大型基因组高效写入的路线图。基因组测序、编辑和合成生物学的进步增强了大规模基因组工程（称为基因组写入）的可行性。在这篇评论文章中，Chari 和 Church 讨论了多种基因组写入策略的优点和局限性，包括广泛修改现有基因组与从头合成基因组，并为写入大型基因组提供了未来的愿景。
Recent exponential advances in genome sequencing and engineering technologies have enabled an unprecedented level of interrogation into the impact of DNA variations (genotype) on cellular function (phenotype). Furthermore, these advances have also prompted realistic discussion of writing and radically re-writing complex genomes. In this Perspective, we detail the motivation for large-scale engineering, discuss the progress made from such projects in bacteria and yeast and describe how various genome-engineering technologies will contribute to this effort. Finally, we describe the features of an ideal platform and provide a roadmap to facilitate the efficient writing of large genomes. Advances in genome sequencing, editing, and synthetic biology have enhanced the feasibility of large-scale genome engineering, termed genome writing. In this Opinion article, Chari and Church discuss the strengths and limitations of diverse strategies for genome writing, including extensively modifying existing genomes versus synthesizing genomes de novo, and they provide future visions for writing large genomes.
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影响因子：
48
作者：
Cai D;Cohen KB;Luo T;Lichtman JW;Sanes JR
通讯作者：
Sanes JR
DOI：
10.1007/978-1-60327-019-9_16
发表时间：
2009-01-01
期刊：
Methods in molecular biology (Clifton, N.J.)
影响因子：
--
作者：
Birling, Marie-Christine;Gofflot, Francoise;Warot, Xavier
通讯作者：
Warot, Xavier
影响因子：
64.8
作者：
Dever DP;Bak RO;Reinisch A;Camarena J;Washington G;Nicolas CE;Pavel-Dinu M;Saxena N;Wilkens AB;Mantri S;Uchida N;Hendel A;Narla A;Majeti R;Weinberg KI;Porteus MH
通讯作者：
Porteus MH
DOI：
10.1126/science.1249252
发表时间：
2014-04-04
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Annaluru N;Muller H;Mitchell LA;Ramalingam S;Stracquadanio G;Richardson SM;Dymond JS;Kuang Z;Scheifele LZ;Cooper EM;Cai Y;Zeller K;Agmon N;Han JS;Hadjithomas M;Tullman J;Caravelli K;Cirelli K;Guo Z;London V;Yeluru A;Murugan S;Kandavelou K;Agier N;Fischer G;Yang K;Martin JA;Bilgel M;Bohutski P;Boulier KM;Capaldo BJ;Chang J;Charoen K;Choi WJ;Deng P;DiCarlo JE;Doong J;Dunn J;Feinberg JI;Fernandez C;Floria CE;Gladowski D;Hadidi P;Ishizuka I;Jabbari J;Lau CY;Lee PA;Li S;Lin D;Linder ME;Ling J;Liu J;Liu J;London M;Ma H;Mao J;McDade JE;McMillan A;Moore AM;Oh WC;Ouyang Y;Patel R;Paul M;Paulsen LC;Qiu J;Rhee A;Rubashkin MG;Soh IY;Sotuyo NE;Srinivas V;Suarez A;Wong A;Wong R;Xie WR;Xu Y;Yu AT;Koszul R;Bader JS;Boeke JD;Chandrasegaran S
通讯作者：
Chandrasegaran S
影响因子：
48
作者：
Agudelo, Daniel;Duringer, Alexis;Doyon, Yannick
通讯作者：
Doyon, Yannick