Synergistic anti-inflammatory effects of nobiletin and sulforaphane in lipopolysaccharide-stimulated RAW 264.7 cells.
Synergistic anti-inflammatory effects of nobiletin and sulforaphane in lipopolysaccharide-stimulated RAW 264.7 cells.
复制标题
DOI：
10.1021/jf300129t
复制
发表时间：
2012-03-07
影响因子：
6.1
通讯作者：
Xiao H
中科院分区：
文献类型：
--
作者：
Guo S;Qiu P;Xu G;Wu X;Dong P;Yang G;Zheng J;McClements DJ;Xiao H
炎症在包括癌症在内的许多疾病的发生和发展中起着重要的作用。在此，我们研究了两种膳食化合物nobiletin （NBN）和sulforaphan （SFN）联合使用的抗炎作用。在脂多糖（LPS）刺激的RAW 264.7巨噬细胞中研究了NBN、SFN及其组合的非细胞毒性浓度。结果表明，在较高浓度下，NBN和SFN联合处理对一氧化氮（NO）生成的抑制作用明显强于NBN或SFN单独处理。根据等线图分析，这些增强的抑制作用是协同的。Western blot分析显示，NBN和SFN联合处理可协同降低iNOS和COX-2蛋白表达水平，诱导血红素加氧酶-1 （HO-1）蛋白表达。Real-time PCR分析显示，低剂量NBN和SFN联合使用可显著抑制lps诱导的IL-1 mRNA水平上调，并协同提高HO-1 mRNA水平。总的来说，我们的研究结果表明，NBN和SFN联合使用在抑制lps诱导的RAW 264.7细胞炎症方面具有协同作用。
Inflammation plays important roles in initiation and progress of many diseases including cancers in multiple organ sites. Herein, we investigated the anti-inflammatory effects of two dietary compounds, nobiletin (NBN) and sulforaphane (SFN) in combination. Non-cytotoxic concentrations of NBN, SFN, and their combinations were studied in lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophage cells. The results showed that combined NBN and SFN treatments produced much stronger inhibitory effects on the production of nitric oxide (NO) than NBN or SFN alone at higher concentrations. These enhanced inhibitory effects were synergistic based on the isobologram analysis. Western blot analysis showed that combined NBN and SFN treatments synergistically decreased iNOS and COX-2 protein expression levels and induced heme oxygenase-1 (HO-1) protein expression. Real-time PCR analysis indicated that low doses of NBN and SFN in combination significantly suppressed LPS-induced upregulation of IL-1 mRNA levels, and synergistically increased HO-1 mRNA levels. Overall our results demonstrated that NBN and SFN in combination produced synergistic effects in inhibiting LPS-induced inflammation in RAW 264.7 cells.
登录
查看更多内容
影响因子：
13.5
作者：
Wang, WZW;Smith, DLH;Zucker, SD
通讯作者：
Zucker, SD
影响因子：
4.4
作者：
Li, N;Alam, J;Nel, AE
通讯作者：
Nel, AE
DOI：
10.1016/0027-5107(94)90245-3
发表时间：
1994-03-01
期刊：
MUTATION RESEARCH
影响因子：
--
作者：
OHSHIMA, H;BARTSCH, H
通讯作者：
BARTSCH, H
影响因子：
5.8
作者：
Lin, Wen;Wu, Rachel T.;Wu, Tienyuan;Khor, Tin-Oo;Wang, Hu;Kong, Ah-Ng
通讯作者：
Kong, Ah-Ng
影响因子：
56.9
作者：
WINK, DA;KASPRZAK, KS;KEEFER, LK
通讯作者：
KEEFER, LK