Standardized environmental enrichment supports enhanced brain plasticity in healthy rats and prevents cognitive impairment in epileptic rats.
Standardized environmental enrichment supports enhanced brain plasticity in healthy rats and prevents cognitive impairment in epileptic rats.
复制标题
DOI：
10.1371/journal.pone.0053888
复制
发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Bezin L
中科院分区：
文献类型：
--
作者：
Fares RP;Belmeguenai A;Sanchez PE;Kouchi HY;Bodennec J;Morales A;Georges B;Bonnet C;Bouvard S;Sloviter RS;Bezin L
实验动物丰富的环境影响大脑的可塑性，刺激神经发生，增加神经营养因子的表达，并防止脑损伤的影响。然而，这些积极的影响并没有经常被观察到，可能是因为缺乏标准化的环境富集程序。因此，我们设计了一个丰富的笼子（Marlau™笼子），它提供：(1)压力最小的社会互动；(2)增加自愿锻炼；(3)多种娱乐活动；(4)认知刺激（迷宫探索）和(5)新奇（迷宫配置每周改变三次）。迷宫将食物颗粒和水瓶分开，保证所有动物的认知刺激。与在传统笼子中饲养的大鼠相比，在Marlau™笼子中饲养的大鼠表现出皮层厚度增加，海马神经发生和海马编码参与组织可塑性和重塑的各种基因的转录物水平增加。此外，Marlau™笼中的大鼠在学习和记忆方面表现出更好的表现，焦虑相关行为减少，急性约束应激后基础血浆皮质酮水平恢复得更好。Marlau™笼还确保空间学习和大脑基因表达分析的实验间再现性。最后，在断奶时严重癫痫持续状态后，将大鼠安置在Marlau™笼子中，可以防止在受到相同侮辱的大鼠中观察到的认知障碍，然后将其安置在传统笼子中。通过在饲养期间为啮齿动物提供标准化的富集环境，Marlau™笼应促进实验室环境富集的统一性。
Environmental enrichment of laboratory animals influences brain plasticity, stimulates neurogenesis, increases neurotrophic factor expression, and protects against the effects of brain insult. However, these positive effects are not constantly observed, probably because standardized procedures of environmental enrichment are lacking. Therefore, we engineered an enriched cage (the Marlau™ cage), which offers: (1) minimally stressful social interactions; (2) increased voluntary exercise; (3) multiple entertaining activities; (4) cognitive stimulation (maze exploration), and (5) novelty (maze configuration changed three times a week). The maze, which separates food pellet and water bottle compartments, guarantees cognitive stimulation for all animals. Compared to rats raised in groups in conventional cages, rats housed in Marlau™ cages exhibited increased cortical thickness, hippocampal neurogenesis and hippocampal levels of transcripts encoding various genes involved in tissue plasticity and remodeling. In addition, rats housed in Marlau™ cages exhibited better performances in learning and memory, decreased anxiety-associated behaviors, and better recovery of basal plasma corticosterone level after acute restraint stress. Marlau™ cages also insure inter-experiment reproducibility in spatial learning and brain gene expression assays. Finally, housing rats in Marlau™ cages after severe status epilepticus at weaning prevents the cognitive impairment observed in rats subjected to the same insult and then housed in conventional cages. By providing a standardized enriched environment for rodents during housing, the Marlau™ cage should facilitate the uniformity of environmental enrichment across laboratories.
登录
查看更多内容
影响因子：
56.9
作者：
Brun, VH;Otnæss, MK;Moser, EI
通讯作者：
Moser, EI
影响因子：
64.5
作者：
Cao L;Liu X;Lin EJ;Wang C;Choi EY;Riban V;Lin B;During MJ
通讯作者：
During MJ
影响因子：
4.2
作者：
Costa, David A.;Craechiolo, Jennifer R.;Potter, Huntington
通讯作者：
Potter, Huntington
影响因子：
2.5
作者：
Cui, Minghu;Yang, Ya;Cao, Jun
通讯作者：
Cao, Jun
影响因子：
2.7
作者：
Cracchiolo, Jennifer R.;Mori, Takashi;Arendash, Gary W.
通讯作者：
Arendash, Gary W.