A Transcriptome-Level Study Identifies Changing Expression Profiles for Ossification of the Ligamentum Flavum of the Spine.
A Transcriptome-Level Study Identifies Changing Expression Profiles for Ossification of the Ligamentum Flavum of the Spine.
复制标题
转录组水平研究确定了脊柱黄韧带骨化的表达谱变化。
DOI：
10.1016/j.omtn.2018.07.018
复制
发表时间：
2018-09-07
期刊：
影响因子：
--
通讯作者：
Xue Y
中科院分区：
文献类型：
--
作者：
Han Y;Hong Y;Li L;Li T;Zhang Z;Wang J;Xia H;Tang Y;Shi Z;Han X;Chen T;Liu Q;Zhang M;Zhang K;Hong W;Xue Y
黄韧带骨化是一种常见的脊柱疾病，可引起脊髓病和神经根病。非编码RNA（ncRNA）参与许多病理过程;然而，很少有ncRNA已被确定为与OLF相关。在这里，我们通过mRNA、lncRNA和circRNA微阵列和microRNA测序比较了OLF患者和健康志愿者OLF组织中lncRNA、mRNA、circRNA和microRNA的表达。在OLF过程中，共有2，054种mRNA，2，567种lncRNA，627种circRNA和28种microRNA（miRNAs）发生了变化。qPCR证实了所选mRNA和ncRNA的差异表达。基于差异表达RNA转录本的相关性分析，构建了lncRNA-mRNA共表达网络、miRNA-mRNA靶标预测网络和circRNA-miRNA-mRNA竞争性内源RNA（ceRNA）网络。随后，对差异表达的mRNA和预测的miRNA靶基因进行基因本体（GO）和京都基因和基因组百科全书（KEGG）途径分析。此外，通过功能获得和功能丧失实验，证实了基于miRNA-19 b-3 p的失调的miRNA-circRNA-lncRNA-mRNA网络在骨化过程中发挥作用。总之，这项研究提供了一个系统的观点，在OLF的发病机制中的ncRNA的潜在功能。
Ossification of the ligamentum flavum (OLF) is a common spinal disorder that causes myelopathy and radiculopathy. Non-coding RNAs (ncRNAs) are involved in numerous pathological processes; however, very few ncRNAs have been identified to be correlated with OLF. Here we compared the expression of lncRNA, mRNA, circRNA, and microRNA in OLF tissues from OLF patients and healthy volunteers through mRNA, lncRNA, and circRNA microarrays and microRNA sequencing. A total of 2,054 mRNAs, 2,567 lncRNAs, 627 circRNAs, and 28 microRNAs (miRNAs) were altered during the process of OLF. qPCR confirmed the differential expression of selected mRNAs and ncRNAs. An lncRNA-mRNA co-expression network, miRNA-mRNA target prediction network, and competing endogenous RNA (ceRNA) network of circRNA-miRNA-mRNA were constructed based on a correlation analysis of the differentially expressed RNA transcripts. Subsequently, gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses for the differentially expressed mRNAs and the predicted miRNAs target genes were performed. In addition, a deregulated miRNA-19b-3p-based miRNA-circRNA-lncRNA-mRNA network was confirmed, by gain-of-function and loss-of-function experiments, to function in the process of ossification. Taken together, this study provides a systematic perspective on the potential function of ncRNAs in the pathogenesis of OLF.
登录
查看更多内容
DOI：
10.1002/jbmr.2287
发表时间：
2014-12
期刊：
Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research
影响因子：
--
作者：
Chen H;Ghori-Javed FY;Rashid H;Adhami MD;Serra R;Gutierrez SE;Javed A
通讯作者：
Javed A
影响因子：
--
作者：
Li F;Zhang L;Li W;Deng J;Zheng J;An M;Lu J;Zhou Y
通讯作者：
Zhou Y
DOI：
10.1056/nejmoa0907705
发表时间：
2010-02-11
期刊：
The New England journal of medicine
影响因子：
--
作者：
Barnes AM;Carter EM;Cabral WA;Weis M;Chang W;Makareeva E;Leikin S;Rotimi CN;Eyre DR;Raggio CL;Marini JC
通讯作者：
Marini JC
影响因子：
2
作者：
Mobbs, Ralph J.;Dvorak, Marcel
通讯作者：
Dvorak, Marcel
影响因子：
20.3
作者：
Khurana, Satish;Buckley, Shannon;Verfaillie, Catherine M.
通讯作者：
Verfaillie, Catherine M.