Progresses of mycobacteriophage-based Mycobacterium tuberculosis detection
Progresses of mycobacteriophage-based Mycobacterium tuberculosis detection
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基于分枝杆菌噬菌体的结核分枝杆菌检测研究进展
DOI：
10.32604/biocell.2020.011713
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发表时间：
2020
期刊：
影响因子：
1.2
通讯作者：
XIANGYU FAN
中科院分区：
文献类型：
--
作者：
ZICHEN LIU;SIYAO GUO;MENGZHI JI;KAILI SUN;ZHONGFANG LI;XIANGYU FAN
结核病 (TB) 仍然是全世界发病和死亡的主要原因，特别是在发展中国家。快速有效的结核病诊断方法对于控制结核病的蔓延趋势至关重要。耐药细菌的出现增加了快速耐药测试的挑战。由于其高特异性和敏感性，基于噬菌体的诊断受到广泛关注。在这篇综述中，我们主要描述了结核病检测中基于分枝杆菌噬菌体的诊断，特别是两种流行的方法：荧光报告噬菌体和噬菌体扩增生物测定（PhaB）。报告噬菌体的原理是携带荧光基因的噬菌体可以特异性感染宿主细菌。噬菌体扩增生物检测的原理是噬菌体在适当的条件下能够感染标本中活的结核杆菌并产生噬菌斑。其他基于噬菌体的诊断方法，例如扩增生物学测定和核酸扩增或侧流测定的组合，也正在积极探索。本综述将有助于我们提高对噬菌体在结核病检测中的认识，更好地推动结核分枝杆菌快速诊断技术的发展。
Tuberculosis (TB) remains a major cause of morbidity and mortality worldwide, particularly in developing countries. A rapid and efficient method for TB diagnosis is indispensable to check the trend of tuberculosis expansion. The emergence of drug-resistant bacteria has increased the challenge of rapid drug resistance tests. Due to its high specificity and sensitivity, bacteriophage-based diagnosis is intensively pursued. In this review, we mainly described mycobacteriophage-based diagnosis in TB detection, especially two prevalent approaches: fluorescent reporter phage and phage amplified biologically assay (PhaB). The rationale of reporter phage is that phage carrying fluorescent genes can infect host bacteria specifically. Phage amplified biological assay based on the principle that phages can infect the live Mycobacterium tuberculosis in the specimen under suitable conditions and produce plaques. Other phage-based diagnostic methods, such as a combination of the amplified biologically assay and nucleic acid amplification or lateral flow assays, are also actively explored. This review will help us improve the understanding of mycobacteriophages in TB detection and better promote the development of the rapid diagnosis of M. tuberculosis.
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DOI：
10.4172/2161-1068.1000214
发表时间：
2016-06
期刊：
Mycobacterial diseases : tuberculosis & leprosy
影响因子：
--
作者：
H. Talwar;J. Talreja;L. Samavati
通讯作者：
H. Talwar;J. Talreja;L. Samavati
影响因子：
9.4
作者：
Christian Carriere;Paul F. Riska;O. Zimhony;J. Kriakov;S. Bardarov;Judah Burns;John Chan;William R. Jacobs
通讯作者：
Christian Carriere;Paul F. Riska;O. Zimhony;J. Kriakov;S. Bardarov;Judah Burns;John Chan;William R. Jacobs
抗砷细菌的噬菌体转导抗性基因，改变砷形态并增加土壤毒性
影响因子：
10.9
作者：
Tang Xiang;Yu Pingfeng;Tang Lin;Zhou Man;Fan Changzheng;Lu Yue;Mathieu Jacques;Xiong Weiping;Alvarez Pedro J.
通讯作者：
Alvarez Pedro J.
影响因子：
5.7
作者：
Swift, Benjamin M. C.;Meade, Nathan;Rees, Catherine E. D.
通讯作者：
Rees, Catherine E. D.
影响因子：
3.7
作者：
Oettinger, T;Holm, A;Haslov, K
通讯作者：
Haslov, K