Urotensin II receptor as a potential biomarker for the prognosis of hepatocellular carcinoma patients.
Urotensin II receptor as a potential biomarker for the prognosis of hepatocellular carcinoma patients.
复制标题
尾加压素 II 受体作为肝细胞癌患者预后的潜在生物标志物
DOI：
10.3892/ol.2017.6545
复制
发表时间：
2017-09
期刊：
影响因子：
2.9
通讯作者：
Ding H
中科院分区：
文献类型：
--
作者：
Wei H;Yu X;Xue X;Liu H;Wang M;Li Y;Wang X;Ding H
尾加压素II及其相关的尾加压素II受体在肝细胞癌的发生中起重要作用。然而，UTR的临床意义仍有待阐明。本研究的目的是调查非翻译区是否显示出作为预测肝癌患者预后的生物标志物的潜力。此外，还研究了非编码区对肝癌细胞运动和侵袭能力的影响。用免疫组织化学方法检测83例既往接受根治性肝切除的肝细胞癌患者的UTR表达水平。分析UTR值与临床病理资料之间的关系。体外用免疫印迹法检测QSG-7701、BEL-7402和MHCC-97H细胞系中UTR的表达。用小干扰(Si)RNA下调BEL-7402和MHCC-97H细胞的非编码区，Transwell比色法检测非编码区对肿瘤细胞运动的影响。UTR的表达与肿瘤数目、大小、组织学类型、肿瘤淋巴结转移/巴塞罗那临床肝癌分期有关。Kaplan-Meier曲线分析显示，UtR表达水平与肝癌患者的无复发和总存活率有关(P<；0.0001)。与QSG-7701相比，体外培养的BEL-7402和MHCC-97H细胞株UTR表达水平升高(P<；0.05)。SiRNA介导的UTR基因沉默显著抑制了BEL-7402和MHCC-97H细胞的运动。提示UTR可作为预测根治性肝切除术后预后的一种新的生物标志物，并可作为抑制肝癌侵袭转移的潜在治疗靶点。
Urotensin II and the associated urotensin II receptor (UTR) are important in the carcinogenesis of hepatocellular carcinoma (HCC). However, the clinical significance of UTR remains to be elucidated. The aim of the present study was to investigate if UTR exhibits the potential to act as a biomarker to predict the prognosis of HCC patients. The effects of UTR on motility and invasion of HCC cells were additionally investigated. UTR expression levels were determined by immunohistochemistry, in 83 HCC patients that previously underwent curative liver resection. The association between UTR levels and clinicopathological data were analyzed. In vitro, the expressions of UTR in QSG-7701, BEL-7402 and MHCC-97H cell lines were determined via western blotting. Small interfering (si)RNA was used to downregulate UTR in BEL-7402 and MHCC-97H cell lines, and the effects of UTR on tumor cell motility were tested by Transwell assay. UTR expression was associated with tumor number, size, histology and tumor node metastasis/Barcelona Clinic Liver Cancer HCC stage. UTR expression levels were additionally associated with recurrence-free and overall survival in HCC patients by Kaplan-Meier curve analysis (P<0.0001). In vitro, UTR expression levels were increased in BEL-7402 and MHCC-97H cell lines, compared with QSG-7701 (P<0.05). siRNA-mediated silencing of the UTR gene significantly inhibited cell motility in BEL-7402 and MHCC-97H cells. The results indicated that UTR may be regarded as a novel biomarker to predict outcomes following radical liver resection and as a potential therapeutic target to inhibit invasion and metastasis of HCC.
登录
查看更多内容
影响因子：
3.7
作者：
Yu X;Luo Y;Liu J;Liu Y;Sun Q
通讯作者：
Sun Q
DOI：
10.12659/msm.890459
发表时间：
2014-08-12
期刊：
Medical science monitor : international medical journal of experimental and clinical research
影响因子：
--
作者：
Balakan O;Kalender ME;Suner A;Cengiz B;Oztuzcu S;Bayraktar R;Borazan E;Babacan T;Camci C
通讯作者：
Camci C
影响因子：
4
作者：
Grieco, Paolo;Franco, Renato;Caraglia, Michele
通讯作者：
Caraglia, Michele
影响因子：
3
作者：
Proulx, Christophe D.;Holleran, Brian J.;Leduc, Richard
通讯作者：
Leduc, Richard
影响因子：
3
作者：
Wang, Hongxia;Dong, Kun;Wang, Xuejiang
通讯作者：
Wang, Xuejiang