Epidemiology of Plasmid Lineages Mediating the Spread of Extended-Spectrum Beta-Lactamases among Clinical Escherichia coli.
Epidemiology of Plasmid Lineages Mediating the Spread of Extended-Spectrum Beta-Lactamases among Clinical Escherichia coli.
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DOI：
10.1128/msystems.00519-22
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发表时间：
2022-10-26
期刊：
影响因子：
6.4
通讯作者：
中科院分区：
文献类型：
--
作者：
广谱β -内酰胺酶（ESBLs）在大肠杆菌临床分离株中的流行率一直在增加，这种传播是由ESBLs编码质粒驱动的。然而，ESBL传播质粒的流行病学研究仍然不足，模糊了单个质粒谱系在ESBL传播中的作用。为了解决这个问题，我们对一家三级保健医院的149株临床esbl样大肠杆菌进行了深入的基因组调查。我们获得了446个质粒的高质量组装，揭示了跨越时间、空间和细菌序列类型边界的质粒共享的广泛图谱。通过基于序列的网络，我们确定了负责主要esbl传播的特定质粒谱系。值得注意的是，我们证明了IncF质粒分为2个不同的谱系，这些谱系为不同的ESBLs富集，并占据不同的宿主范围。我们的工作提供了质粒介导的ESBLs传播的详细图片，展示了鉴定谱系中广泛的序列多样性，同时强调了这些质粒在临床大肠杆菌群体中持续存在的遗传因素。考虑到可用于此类感染的治疗方案有限，产广谱β -内酰胺酶（ESBL）的大肠杆菌的医院感染发生率不断上升，对公众健康构成了重大威胁。ESBL的快速传播被认为是由共轭质粒上的抗性基因定位驱动的。在这里，我们确定了不同质粒谱系在ESBLs医院传播中的贡献。我们为质粒介导的ESBL传播提供了进一步的支持，但证明了一些ESBL基因比其他基因更依赖于质粒传播。我们确定了富含主要ESBL基因的关键质粒谱系，并强调了促进这些质粒群在临床大肠杆菌群体中传播和稳定维持的编码遗传元件。总的来说，我们的工作为ESBLs通过质粒传播提供了有价值的见解，进一步加深了我们对这些基因在医院环境中患病率增加的潜在因素的理解。
The prevalence of extended-spectrum beta-lactamases (ESBLs) among clinical isolates of Escherichia coli has been increasing, with this spread driven by ESBL-encoding plasmids. However, the epidemiology of ESBL-disseminating plasmids remains understudied, obscuring the roles of individual plasmid lineages in ESBL spread. To address this, we performed an in-depth genomic investigation of 149 clinical ESBL-like E. coli isolates from a tertiary care hospital. We obtained high-quality assemblies for 446 plasmids, revealing an extensive map of plasmid sharing that crosses time, space, and bacterial sequence type boundaries. Through a sequence-based network, we identified specific plasmid lineages that are responsible for the dissemination of major ESBLs. Notably, we demonstrate that IncF plasmids separate into 2 distinct lineages that are enriched for different ESBLs and occupy distinct host ranges. Our work provides a detailed picture of plasmid-mediated spread of ESBLs, demonstrating the extensive sequence diversity within identified lineages, while highlighting the genetic elements that underlie the persistence of these plasmids within the clinical E. coli population. IMPORTANCE The increasing incidence of nosocomial infections with extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli represents a significant threat to public health, given the limited treatment options available for such infections. The rapid ESBL spread is suggested to be driven by localization of the resistance genes on conjugative plasmids. Here, we identify the contributions of different plasmid lineages in the nosocomial spread of ESBLs. We provide further support for plasmid-mediated spread of ESBLs but demonstrate that some ESBL genes rely on dissemination through plasmids more than the others. We identify key plasmid lineages that are enriched in major ESBL genes and highlight the encoded genetic elements that facilitate the transmission and stable maintenance of these plasmid groups within the clinical E. coli population. Overall, our work provides valuable insight into the dissemination of ESBLs through plasmids, furthering our understating of factors underlying the increased prevalence of these genes in nosocomial settings.
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影响因子：
2.1
作者：
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影响因子：
4.5
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de Been M;Lanza VF;de Toro M;Scharringa J;Dohmen W;Du Y;Hu J;Lei Y;Li N;Tooming-Klunderud A;Heederik DJ;Fluit AC;Bonten MJ;Willems RJ;de la Cruz F;van Schaik W
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van Schaik W
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Walsh, Timothy R.
影响因子：
5.8
作者：
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通讯作者：
Robles, M