Hydrogen peroxide release by rat peritoneal macrophages in the presence and absence of tumor cells.
Hydrogen peroxide release by rat peritoneal macrophages in the presence and absence of tumor cells.
复制标题
在肿瘤细胞存在和不存在的情况下，大鼠腹膜巨噬细胞释放过氧化氢。
DOI：
10.1016/0003-9861(82)90096-0
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发表时间：
1982
影响因子：
3.9
通讯作者：
Fishman,M
中科院分区：
文献类型：
--
作者：
Thomas,EL;Fishman,M
在组织培养的大鼠淋巴瘤细胞存在和不存在的情况下，以及在佛波酯(PMA)存在的情况下，测定了矿物油诱导的大鼠腹膜巨噬细胞向细胞外介质释放过氧化氢(H_2O_2)的能力。用辣根过氧化物酶(HRP)催化氧化东莨内酯或酚红来测定细胞在单层培养中长达24 h的H_2O_2释放量，无论有或没有PMA，巨噬细胞都能释放H_2O_2，而PMA在短时间(1~4 h)可显著增加H_2O_2的释放量。肿瘤细胞没有取代PMA作为过氧化氢释放的触发剂。相反，肿瘤细胞抑制了过氧化氢的释放。抑制的可能基础是巨噬细胞和肿瘤细胞之间竞争供氧(O2)。当O2浓度保持不变时，肿瘤细胞不抑制H2O2的释放。用O2电极测量，巨噬细胞摄取O2和释放H2O2的速率与O2浓度成正比。在两种不同的细胞外H_2O_2消耗系统(HRP-东五味子内酯和过氧化氢酶)存在下，H_2O_2的释放速率可以根据测得的氧吸收速率常数的差异来计算。PMA刺激的O2摄取和H2O2释放在一小部分巨噬细胞中最高，在牛血清白蛋白梯度上密度最低的位置获得。在没有PMA的情况下，这些细胞也释放了过氧化氢。肿瘤细胞对未分离的巨噬细胞或巨噬细胞亚群摄取和释放O2的速率常数没有影响。
The ability of mineral oil-elicited rat peritoneal macrophages to release hydrogen peroxide (H2O2) to the extracellular medium was measured in the presence and absence of rat lymphoma cells grown in tissue culture, and in the presence of phorbol myristate acetate (PMA). Horseradish peroxidase (HRP)-catalyzed oxidation of scopoletin or phenol red was used to measure H2O2release during incubation of cells in monolayer culture for periods up to 24 h. Macrophages appeared to release H2O2with or without PMA, although PMA greatly increased the amount of H2O2released in short (1 to 4 h) incubations. Tumor cells did not replace PMA as a triggering agent for H2O2release. Instead, tumor cells inhibited H2O2release. The probable basis for inhibition was competition between macrophages and tumor cells for the supply of oxygen (O2). Tumor cells did not inhibit H2O2release when the O2concentration was held constant. The rates at which macrophages took up O2and released H2O2were proportional to the O2concentration, as measured with the O2electrode. Rates of H2O2release could be calculated from the difference in the rate constants for O2uptake measured in the presence of two different extracellular H2O2-consuming systems (HRP-scopoletin vs catalase). PMA-stimulated uptake of O2and release of H2O2were highest in a small subpopulation of macrophages, obtained at the lowest-density position on gradients of bovine serum albumin. These cells also released H2O2in the absence of PMA. Tumor cells had no effect on the rate constants for O2uptake and H2O2release by the unfractionated macrophages or the macrophage subpopulations.
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影响因子：
--
作者：
Westergaard MW;Draborg AH;Troelsen L;Jacobsen S;Houen G
通讯作者：
Houen G
DOI：
10.4103/0973-029x.174643
发表时间：
2015-09
期刊：
Journal of oral and maxillofacial pathology : JOMFP
影响因子：
--
作者：
Adtani P;Malathi N
通讯作者：
Malathi N
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影响因子：
--
作者：
V. S. Negi;N. Muralidharan;S. Mehra;P. Devaraju;C. Mariaselvam;C. Mariaselvam;R. Gulati;S. Salah;C. Fortier;Dominique Charron;Rajagopal Krishnamoorthy;Ryad Tamouza
通讯作者：
Ryad Tamouza
影响因子：
2.2
作者：
Erre, Gian Luca;Mameli, Giuseppe;Sechi, Leonardo Antonio
通讯作者：
Sechi, Leonardo Antonio
影响因子：
5.8
作者：
Cossu, Davide;Mameli, Giuseppe;Sechi, Leonardo Antonio
通讯作者：
Sechi, Leonardo Antonio