Physiologic significance of epithelial removal on guinea pig tracheal smooth muscle response to acetylcholine and serotonin.
Physiologic significance of epithelial removal on guinea pig tracheal smooth muscle response to acetylcholine and serotonin.
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上皮去除对豚鼠气管平滑肌对乙酰胆碱和血清素反应的生理意义。
DOI：
10.1164/ajrccm/147.6_pt_1.1477
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发表时间：
1993
期刊：
影响因子：
--
通讯作者：
Mitchell,RW
中科院分区：
文献类型：
--
作者：
Strek,ME;White,SR;Ndukwu,IM;Munoz,NM;Williams,FS;Vita,AJ;Leff,AR;Mitchell,RW
用戊巴比妥（50 mg/kg腹腔内）麻醉体重为500 - 800 g的雄性Hartley豚鼠，并进行低位颈部气管切开术。使用恒容呼吸机（型号683;哈佛仪器公司，South Natick，MA）用纯氧连续给动物通气，使Pa 02超过300 mm Hg。潮气量设定为6 ml/kg，呼吸频率设定为60次/min，以维持Pac 02在35 - 45 mm Hg之间，动脉血pH在7. 5和7.45（22，23）。在气管造口术部位近端准备一段1.0 cm的颈段气管用于等距固定。该制剂保留了离体节段内平滑肌的循环和神经支配（22，23）。气管段通过缝线连接到一侧的平行杆和力位移传感器（FT型）。03;草仪器，昆西，马萨诸塞州）在另一个。通过从给予激动剂后获得的总力减去气管段的初始静息（被动）力来确定主动收缩力。通过除以单个节段（22，23）的纵向长度将主动力归一化，并表示为主动张力（AT），单位为克力/厘米纵向长度（g/cm）。在这些实验中，主动张力可以分解为< 0.04 g/cm（22）。静息张力设定为10 g/cm，该张力对应于发生最大可再现收缩的气管段的最佳静息长度（Lmax）（22）。
Male Hartley guinea pigs weighing 500 to 800 g were anesthetized with pentobarbital (50 mg/kg intraperitoneally), and a low cervical tracheostomy was performed. Animals were ventilated continuously with pure oxygen using a constant-volume ventilator (Model 683; Harvard Instruments, South Natick, MA), so that Pa02 exceeded 300 mm Hg. Tidal volume was set at 6 ml/kg, and respiratory rate was set to 60 breaths/min to maintain Pac02between 35 and 45 mm Hg and arterial blood pH between 7.~ 5 and 7.45 (22, 23).A 1.0 em segment of cervical trachea was prepared for isometric fixation proximal to the tracheostomy site. This preparation preserves circulation and innervation to the smooth muscle within the isolated segment (22, 23). The tracheal segment was attached by sutures to a parallel bar on one side and a force displacement transducer (Model FT. 03; Grass Instruments, QUincy, MA) on the other. Active force of contraction was determined by SUbtracting initial resting (passive) force of the tracheal segment from total force achieved after administration of agonist. Active force was normalized by dividing by the longitudinal length of the individual segment (22, 23) and expressed as active tension (AT) in grams force per centimeter longitudinal length (g/cm). Active tension could be resolved to< 0.04 g/cm in these experiments (22). Resting tension was set to 10 g/cm, the tension that corresponds to the optimal resting length of the tracheal segment (Lmax) from which maximal, reproducible contraction occurs (22).
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DOI：
10.1152/jappl.1987.63.6.2510
发表时间：
1987
期刊：
Journal of applied physiology (Bethesda, Md. : 1985)
影响因子：
--
作者：
Stuart-Smith,K;Vanhoutte,PM
通讯作者：
Vanhoutte,PM
上皮去除对猫和豚鼠离体中央气道中非肾上腺素能、非胆碱能抑制反应的影响。
影响因子：
5
作者：
Thompson,DC;Wells,JL;Altiere,RJ;Diamond,L
通讯作者：
Diamond,L
上皮去除改变了豚鼠气管对 P 物质的反应性。
DOI：
10.1152/jappl.1989.66.1.232
发表时间：
1989
期刊：
Journal of applied physiology (Bethesda, Md. : 1985)
影响因子：
--
作者：
Fine,JM;Gordon,T;Sheppard,D
通讯作者：
Sheppard,D
上皮在气道平滑肌对松弛激动剂的反应中的作用。
DOI：
10.1152/jappl.1991.71.4.1434
发表时间：
1991
期刊：
Journal of applied physiology (Bethesda, Md. : 1985)
影响因子：
--
作者：
Yang,JN;Mitzner,W;Hirshman,C
通讯作者：
Hirshman,C
上皮调节气管平滑肌对抗原刺激的反应。
影响因子：
3.3
作者：
N. Frossard;F. Muller
通讯作者：
F. Muller