In vitro blood-brain barrier models: current and perspective technologies.
In vitro blood-brain barrier models: current and perspective technologies.
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DOI：
10.1002/jps.23022
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发表时间：
2012-04
影响因子：
3.8
通讯作者：
Cucullo, Luca
中科院分区：
文献类型：
--
作者：
Naik, Pooja;Cucullo, Luca
关键词：
即使在21世纪，旨在描述与中枢神经系统疾病的发生和进展相关的病理范式的研究主要是在实验动物中进行的。然而，有限的翻译意义，高成本，以及开发适当模型（例如，转基因或近交系）的劳动力倾向于平行的体外方法。体外模型对血脑屏障（BBB）的脑血管研究特别有意义，因为血脑屏障在维持大脑稳态和神经元功能方面起着关键作用。由于血脑屏障对许多与流变学和系统性损伤（如灌注不足）相关的事件（包括暴露于潜在有害的外源药物）做出动态反应，因此开发能够复制脑微血管血管特性的更复杂的人工系统正成为基础、转化和药物研究的主要焦点。体外血脑屏障模型是有价值且易于使用的辅助工具，可以在动物和人类研究之前进行和补充。在本文中，我们提供了一个详细的回顾和分析目前可用的体外血脑屏障模型，从静态培养系统到最先进的基于流动和三维共培养装置。我们还讨论了这个不断扩大的研究领域的最新发展和前景。
Even in the 21st century, studies aimed at characterizing the pathological paradigms associated with the development and progression of central nervous system diseases are primarily performed in laboratory animals. However, limited translational significance, high cost, and labor to develop the appropriate model (e.g., transgenic or inbred strains) have favored parallel in vitro approaches. In vitro models are of particular interest for cerebrovascular studies of the blood–brain barrier (BBB), which plays a critical role in maintaining the brain homeostasis and neuronal functions. Because the BBB dynamically responds to many events associated with rheological and systemic impairments (e.g., hypoperfusion), including the exposure of potentially harmful xenobiotics, the development of more sophisticated artificial systems capable of replicating the vascular properties of the brain microcapillaries are becoming a major focus in basic, translational, and pharmaceutical research. In vitro BBB models are valuable and easy to use supporting tools that can precede and complement animal and human studies. In this article, we provide a detailed review and analysis of currently available in vitro BBB models ranging from static culture systems to the most advanced flow-based and three-dimensional coculture apparatus. We also discuss recent and perspective developments in this ever expanding research field.
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影响因子：
3.5
作者：
Aschner, M
通讯作者：
Aschner, M
DOI：
10.1023/a:1011965307612
发表时间：
2001-01-01
期刊：
JOURNAL OF NEUROCYTOLOGY
影响因子：
--
作者：
Bandopadhyay, R;Orte, C;Allt, G
通讯作者：
Allt, G
DOI：
10.1159/000264398
发表时间：
2010-01-01
期刊：
FRONTIERS IN EATING AND WEIGHT REGULATION
影响因子：
--
作者：
Banks, William A.
通讯作者：
Banks, William A.
影响因子：
3
作者：
BALLERMANN, BJ;OTT, MJ
通讯作者：
OTT, MJ
影响因子：
6.5
作者：
ADES, EW;CANDAL, FJ;LAWLEY, TJ
通讯作者：
LAWLEY, TJ