Microarray analysis of microRNA expression in the developing mammalian brain.
Microarray analysis of microRNA expression in the developing mammalian brain.
复制标题
DOI：
10.1186/gb-2004-5-9-r68
复制
发表时间：
2004
期刊：
影响因子：
12.3
通讯作者：
Horvitz HR
中科院分区：
文献类型：
--
作者：
Miska EA;Alvarez-Saavedra E;Townsend M;Yoshii A;Sestan N;Rakic P;Constantine-Paton M;Horvitz HR
一种微阵列技术，适合于分析的microRNA和其他小RNA的表达被用来确定在小鼠大脑发育过程中的microRNA表达谱，并观察到的时序类microRNA的基因表达的时间波。MicroRNA是一类在线虫、植物、昆虫和哺乳动物中发现的新的微小调控RNA。MicroRNA被认为是基因表达的转录后调节因子。在无脊椎动物中，microRNA被认为是发育时间、神经元分化、细胞增殖、程序性细胞死亡和脂肪代谢的调节因子。关于microRNA在哺乳动物中的作用知之甚少。我们从发育中的大鼠和猴脑中分离出18-26个核苷酸的RNA。根据这些RNA的序列以及大鼠和人类基因组的序列，我们确定了这些小RNA中哪些可能来自典型的microRNA的茎环前体。接下来，我们开发了一种适合检测microRNA的微阵列技术，并打印了一个microRNA微阵列，代表了138种哺乳动物microRNA，与我们克隆的microRNA序列以及其他已知的microRNA序列相对应。我们使用这种微阵列来确定发育中的小鼠大脑中表达的microRNA的概况。我们观察到microRNA表达的时间波，表明microRNA在哺乳动物大脑发育中起重要作用。我们描述了一种微阵列技术，可用于分析microRNA和其他小RNA的表达。microRNA微阵列提供了一个新的工具，应该促进microRNA的生物学作用的研究。我们使用这种方法来确定小鼠大脑发育过程中的microRNA表达谱，并观察到顺序类microRNA的基因表达的时间波。
A microarray technology suitable for analyzing the expression of microRNAs and of other small RNAs was used to determine the microRNA expression profile during mouse-brain development and observed a temporal wave of gene expression of sequential classes of microRNAs. MicroRNAs are a large new class of tiny regulatory RNAs found in nematodes, plants, insects and mammals. MicroRNAs are thought to act as post-transcriptional modulators of gene expression. In invertebrates microRNAs have been implicated as regulators of developmental timing, neuronal differentiation, cell proliferation, programmed cell death and fat metabolism. Little is known about the roles of microRNAs in mammals. We isolated 18-26 nucleotide RNAs from developing rat and monkey brains. From the sequences of these RNAs and the sequences of the rat and human genomes we determined which of these small RNAs are likely to have derived from stem-loop precursors typical of microRNAs. Next, we developed a microarray technology suitable for detecting microRNAs and printed a microRNA microarray representing 138 mammalian microRNAs corresponding to the sequences of the microRNAs we cloned as well as to other known microRNAs. We used this microarray to determine the profile of microRNAs expressed in the developing mouse brain. We observed a temporal wave of expression of microRNAs, suggesting that microRNAs play important roles in the development of the mammalian brain. We describe a microarray technology that can be used to analyze the expression of microRNAs and of other small RNAs. MicroRNA microarrays offer a new tool that should facilitate studies of the biological roles of microRNAs. We used this method to determine the microRNA expression profile during mouse brain development and observed a temporal wave of gene expression of sequential classes of microRNAs.
登录
查看更多内容
影响因子：
14.9
作者：
Griffiths-Jones, S
通讯作者：
Griffiths-Jones, S
影响因子：
14.9
作者：
Gupta, V;Cherkassky, A;DiMeo, J
通讯作者：
DiMeo, J
影响因子：
56.9
作者：
AMBROS, V;HORVITZ, HR
通讯作者：
HORVITZ, HR
影响因子：
11.6
作者：
Aukerman, MJ;Sakai, H
通讯作者：
Sakai, H
DOI：
10.1073/pnas.2333854100
发表时间：
2004-01-06
影响因子：
11.1
作者：
Kim, J;Krichevsky, A;Ruvkun, G
通讯作者：
Ruvkun, G